Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- PA5-53860 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EDARADD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PTISDLLNDQ DLLDVIRIKL DPCHPTVKNW RNFASKWGMS YDELCFLEQR PQSPTLEFLL RNSQRTVGQL MELCRLYHRA DVEKVLRRWV DEEWPKRERG DP Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 91%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EDARADD in control (vector only transfected HEK293T lysate) and EDARADD over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a EDARADD Polyclonal Antibody (Product # PA5-53860).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of EDARADD in human skin using EDARADD Polyclonal Antibody (Product # PA5-53860) shows weak cytoplasmic positivity in squamous epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of EDARADD in human placenta using EDARADD Polyclonal Antibody (Product # PA5-53860) shows strong cytoplasmic positivity in trophoblastic cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of EDARADD in human urinary bladder using EDARADD Polyclonal Antibody (Product # PA5-53860) shows strong cytoplasmic positivity in urothelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of EDARADD in human prostate using EDARADD Polyclonal Antibody (Product # PA5-53860) shows moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of EDARADD in human skeletal muscle using EDARADD Polyclonal Antibody (Product # PA5-53860) shows low positivity in myocytes as expected.