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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [2]
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- Product number
- NB300-233 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-233, RRID:AB_10001313
- Product name
- Rabbit Polyclonal TAO2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~120k TAO2 phosphorylated at Ser181 in Western blots. Immunolabeling is completely eliminated by treatment with lambda phosphatase.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references TAO (thousand-and-one amino acid) protein kinases mediate signaling from carbachol to p38 mitogen-activated protein kinase and ternary complex factors.
Regulation of stress-responsive mitogen-activated protein (MAP) kinase pathways by TAO2.
Isolation of the protein kinase TAO2 and identification of its mitogen-activated protein kinase/extracellular signal-regulated kinase kinase binding domain.
Isolation of the protein kinase TAO2 and identification of its mitogen-activated protein kinase/extracellular signal-regulated kinase kinase binding domain.
Chen Z, Raman M, Chen L, Lee SF, Gilman AG, Cobb MH
The Journal of biological chemistry 2003 Jun 20;278(25):22278-83
The Journal of biological chemistry 2003 Jun 20;278(25):22278-83
Regulation of stress-responsive mitogen-activated protein (MAP) kinase pathways by TAO2.
Chen Z, Cobb MH
The Journal of biological chemistry 2001 May 11;276(19):16070-5
The Journal of biological chemistry 2001 May 11;276(19):16070-5
Isolation of the protein kinase TAO2 and identification of its mitogen-activated protein kinase/extracellular signal-regulated kinase kinase binding domain.
Chen Z, Hutchison M, Cobb MH
The Journal of biological chemistry 1999 Oct 1;274(40):28803-7
The Journal of biological chemistry 1999 Oct 1;274(40):28803-7
Isolation of the protein kinase TAO2 and identification of its mitogen-activated protein kinase/extracellular signal-regulated kinase kinase binding domain.
Chen Z, Hutchison M, Cobb MH
The Journal of biological chemistry 1999 Oct 1;274(40):28803-7
The Journal of biological chemistry 1999 Oct 1;274(40):28803-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TAO2 [p Ser181] Antibody [NB300-233] - Western blot of rat hippocampal lysate showing specific labeling of the ~120 kDa TAO Kinase 2 (phospho-Ser181) band.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TAO2 [p Ser181] Antibody [NB300-233] - Western blot of rat cortex lysate showing specific immunolabeling of the ~120k TAO2 phosphorylated at Ser181 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubatede (1200 units for 30 min) before being exposed to the Ser181 TAO2 antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
