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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- PA5-55070 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADSS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KLDGEIIPHI PANQEVLNKV EVQYKTLPGW NTDISNARAF KELPVNAQNY VRFIEDELQI PVKWIGVGKS RESM
- Concentration
- 0.1 mg/mL
Submitted references Proteomic analysis of the effect of the polyphenol pentagalloyl glucose on proteins involved in neurodegenerative diseases in activated BV‑2 microglial cells.
Mendonca P, Taka E, Soliman KFA
Molecular medicine reports 2019 Aug;20(2):1736-1746
Molecular medicine reports 2019 Aug;20(2):1736-1746
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ADSS in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using an ADSS Polyclonal Antibody (Product # PA5-55070).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ADSS in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4. Samples were probed using an ADSS Polyclonal Antibody (Product # PA5-55070).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ADSS in human cell line U-251 MG shows positivity in plasma membrane & cytoplasm. Samples were probed using an ADSS Polyclonal Antibody (Product # PA5-55070).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ADSS in human testis and skeletal muscle tissues using ADSS Polyclonal Antibody (Product # PA5-55070). Corresponding ADSS RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ADSS in human prostate using ADSS Polyclonal Antibody (Product # PA5-55070) shows moderate cytoplasmic and membranous positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ADSS in human rectum using ADSS Polyclonal Antibody (Product # PA5-55070) shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ADSS in human skeletal muscle using ADSS Polyclonal Antibody (Product # PA5-55070) shows weak cytoplasmic positivity in myocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ADSS in human testis using ADSS Polyclonal Antibody (Product # PA5-55070) shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5. Effect of PGG on the expression of ADSS as determined by western analysis with automated capillary electrophoresis. (A) Graph presenting the chemiluminescence levels measured in the different treatments. (B) shows bands representing the protein expression in control (DMSO), PGG (25 uM), LPS/IFNgamma, and pretreated cells (PGG + LPS/IFNgamma) after 24 h of treatment (n=3). ADSS, adenylosuccinate synthetase isozyme 2; PPG, polyphenol pentagalloyl glucose (1,2,3,4,6-penta-O-galloyl-beta-D-glucose); LPS, lipopolysaccharide; IFNgamma, interferon gamma; MW, molecular weight.
