PA5-55070

antibody from Invitrogen Antibodies

Targeting: ADSS2 ADSS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-55070

Provider

Invitrogen Antibodies

Product name

ADSS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: KLDGEIIPHI PANQEVLNKV EVQYKTLPGW NTDISNARAF KELPVNAQNY VRFIEDELQI PVKWIGVGKS RESM Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 96%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Proteomic analysis of the effect of the polyphenol pentagalloyl glucose on proteins involved in neurodegenerative diseases in activated BV‑2 microglial cells.

Mendonca P, Taka E, Soliman KFA
Molecular medicine reports 2019 Aug;20(2):1736-1746

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ADSS in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using an ADSS Polyclonal Antibody (Product # PA5-55070).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ADSS in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4. Samples were probed using an ADSS Polyclonal Antibody (Product # PA5-55070).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of ADSS in human cell line U-251 MG shows positivity in plasma membrane & cytoplasm. Samples were probed using an ADSS Polyclonal Antibody (Product # PA5-55070).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ADSS in human testis and skeletal muscle tissues using ADSS Polyclonal Antibody (Product # PA5-55070). Corresponding ADSS RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ADSS in human prostate using ADSS Polyclonal Antibody (Product # PA5-55070) shows moderate cytoplasmic and membranous positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ADSS in human rectum using ADSS Polyclonal Antibody (Product # PA5-55070) shows moderate to strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ADSS in human skeletal muscle using ADSS Polyclonal Antibody (Product # PA5-55070) shows weak cytoplasmic positivity in myocytes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ADSS in human testis using ADSS Polyclonal Antibody (Product # PA5-55070) shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5. Effect of PGG on the expression of ADSS as determined by western analysis with automated capillary electrophoresis. (A) Graph presenting the chemiluminescence levels measured in the different treatments. (B) shows bands representing the protein expression in control (DMSO), PGG (25 uM), LPS/IFNgamma, and pretreated cells (PGG + LPS/IFNgamma) after 24 h of treatment (n=3). ADSS, adenylosuccinate synthetase isozyme 2; PPG, polyphenol pentagalloyl glucose (1,2,3,4,6-penta-O-galloyl-beta-D-glucose); LPS, lipopolysaccharide; IFNgamma, interferon gamma; MW, molecular weight.