Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AM00060PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM00060PU-N, RRID:AB_981240
- Product name
- anti FER / TYK3 (incl. pos. control)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide conjugated to KLH.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6B9
- Vial size
- 0.1 mg
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Detection of endogenous fer: Whole cell extracts of pervanadate (VH) treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab fer-6B9 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). VH treatment: 15 min. Lane 1: A431; Lane 2: SW480 lane 3: SW620; lane 4: HT29; lane 5: MCF-7; lane 6: MDA-MB231; lane 7: T47D