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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00000814-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000814-M01, RRID:AB_425341
- Product name
- CAMK4 monoclonal antibody (M01), clone 1A3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant CAMK4.
- Antigen sequence
MLKVTVPSCSASSCSSVTASAAPGTASLVPDYWID
GSNRDALSDFFEVESELGRGATSIVYRCKQKGTQK
PYALKVLKKTVDKKIVRTEIGVLLRLSHPNIIKLK
EIFETPTEISLVLELVTGGELFDRIVEKGYYSERD
AADAVKQILEAVAYLHENGIVHRDLKPENLLYATP
APDAPLKIADFGLSKIVEHQVLMKTVCGTPGYCAP
EILRGCAYGPEVDMWSVGIITYILLCGFEPFYDER
GDQFMFRRILNCEYYFISPWWDEVSLNAKDLVRKL
IVLDPKKRLTTFQALQHPWVTGKAANFVHMDTAQK
KLQEFNARRKLKAAVKAVVASSRLGSASSSHGSIQ
ESHKASRDPSPIQDGNEDMKAIPEGEKIQGDGAQA
AVKGAQAELMKVQALEKVKGADINAEEAPKMVPKA
VEDGIKVADLELEEGLAEEKLKTVEEAAAPREGQG
SSAVGFEVPQQDVILPEY- Isotype
- IgG
- Antibody clone number
- 1A3
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Acid activation of Trpv1 leads to an up-regulation of calcitonin gene-related peptide expression in dorsal root ganglion neurons via the CaMK-CREB cascade: a potential mechanism of inflammatory pain.
Nakanishi M, Hata K, Nagayama T, Sakurai T, Nishisho T, Wakabayashi H, Hiraga T, Ebisu S, Yoneda T
Molecular biology of the cell 2010 Aug 1;21(15):2568-77
Molecular biology of the cell 2010 Aug 1;21(15):2568-77
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CAMK4 monoclonal antibody (M01), clone 1A3 Western Blot analysis of CAMK4 expression in Jurkat ( Cat # L017V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of CAMK4 expression in transfected 293T cell line by CAMK4 monoclonal antibody (M01), clone 1A3.Lane 1: CAMK4 transfected lysate(52.14 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to CAMK4 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to CAMK4 on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
