PAB9938

antibody from Abnova Corporation

Targeting: CAMK4 CaMK-GR, CaMKIV

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB9938

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB9938, RRID:AB_1672184

Product name

CAMK4 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of CAMK4.

Storage

Store at 4°C on dry atmosphere.After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The cDNA sequence and characterization of the Ca2+/calmodulin-dependent protein kinase-Gr from human brain and thymus.

Bland MM, Monroe RS, Ohmstede CA
Gene 1994 May 16;142(2):191-7

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A Ca2+/calmodulin-dependent protein kinase, CaM kinase-Gr, expressed after transformation of primary human B lymphocytes by Epstein-Barr virus (EBV) is induced by the EBV oncogene LMP1.

Mosialos G, Hanissian SH, Jawahar S, Vara L, Kieff E, Chatila TA
Journal of virology 1994 Mar;68(3):1697-705

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cDNA cloning and expression of human calmodulin-dependent protein kinase IV.

Kitani T, Okuno S, Fujisawa H
Journal of biochemistry 1994 Apr;115(4):637-40

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using CAMK4 polyclonal antibody (Cat # PAB9938) shows detection of a band ~52 KDa corresponding to CAMK4 (arrowhead) in various preparations.Staining ofrat brain lysate is shown in lane 1.Jurkat cell lysate staining is shown in lane 2.Specific reactivity is blocked in both lysates when antibodyis preincubated with immunizing peptide (lanes 3 and 4 respectively).Approximately 35 ug of each lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.CAMK4 was similarly detected on lysates from mouse brain (not shown).After blocking the membrane was probed with the primary antibody diluted to 1 : 1,000 for 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L]MX for 45 min at room temperature.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining with CAMK4 polyclonal antibody (Cat # PAB9938) was diluted 1 : 500 to detect CAMK4 in human brain cortex tissue.Tissue was formalin fixed and paraffin embedded.No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)