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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- A300-494A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CamK4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CamK4 by western blot and immunoprecipitation. Samples: A) Whole cell lysate from Jurkat (5, 15 and 50 µg) and HeLa (50 µg) cells. B) Whole cell lysate (1 mg/IP; 1/4 of IP loaded/lane) from Jurkat cells. Antibodies: Affinity purified rabbit anti-CamK4 antibody BL1836 (Cat. No. A300-494A) used at 0.04 µg/ml for WB (A and B) and at 3 µg/mg lysate for IP (B). CamK4 was also immunoprecipitated using CamK4 antibodies BL1834 (Cat. No. A300-492A) and BL1835 (Cat. No. A300-493A) used at 3 µg/mg lysate for IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CanK4 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CamK4 (Cat. No. A300-494A) used at a dilution of 1:1,000 (0.2µg/ml). Detection: DAB.
