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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PAB0624 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0624, RRID:AB_983043
- Product name
- TNIK (phospho S764) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of TNIK.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The Traf2- and Nck-interacting kinase as a putative effector of Rap2 to regulate actin cytoskeleton.
TNIK, a novel member of the germinal center kinase family that activates the c-Jun N-terminal kinase pathway and regulates the cytoskeleton.
Antisense display--a method for functional gene screening: evaluation in a cell-free system and isolation of angiogenesis-related genes.
Taira K, Umikawa M, Takei K, Myagmar BE, Shinzato M, Machida N, Uezato H, Nonaka S, Kariya K
The Journal of biological chemistry 2004 Nov 19;279(47):49488-96
The Journal of biological chemistry 2004 Nov 19;279(47):49488-96
TNIK, a novel member of the germinal center kinase family that activates the c-Jun N-terminal kinase pathway and regulates the cytoskeleton.
Fu CA, Shen M, Huang BC, Lasaga J, Payan DG, Luo Y
The Journal of biological chemistry 1999 Oct 22;274(43):30729-37
The Journal of biological chemistry 1999 Oct 22;274(43):30729-37
Antisense display--a method for functional gene screening: evaluation in a cell-free system and isolation of angiogenesis-related genes.
Yonekura H, Migita H, Sakurai S, Wang H, Harada S, Abedin MJ, Yamagishi S, Yamamoto H
Nucleic acids research 1999 Jul 1;27(13):2591-600
Nucleic acids research 1999 Jul 1;27(13):2591-600
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with TNIK (phospho S764) polyclonal antibody (Cat # PAB0624) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot blot analysis of TNIK (phospho S764) polyclonal antibody (Cat # PAB0624) and TNIK-pS764 Pab on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5 ug/mL. P-Pab : phosphorylated antibody; NP-Pab : non-phophorylated antibody; P-Pep : phospho-peptide; NP-Pep : non-phospho-peptide.
- Validation comment
- Dot Blot (Peptide)
