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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-15181 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TNIK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A null mutation in TNIK defines a novel locus for intellectual disability.
Organization of TNIK in dendritic spines.
Anazi S, Shamseldin HE, AlNaqeb D, Abouelhoda M, Monies D, Salih MA, Al-Rubeaan K, Alkuraya FS
Human genetics 2016 Jul;135(7):773-8
Human genetics 2016 Jul;135(7):773-8
Organization of TNIK in dendritic spines.
Burette AC, Phend KD, Burette S, Lin Q, Liang M, Foltz G, Taylor N, Wang Q, Brandon NJ, Bates B, Ehlers MD, Weinberg RJ
The Journal of comparative neurology 2015 Sep 1;523(13):1913-24
The Journal of comparative neurology 2015 Sep 1;523(13):1913-24
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TNIK using a TNIK polyclonal antibody (Product # PA5-15181) in mouse brain tissue lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a TNIK polyclonal antibody (Product # PA5-15181) in HeLa cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a TNIK polyclonal antibody (Product # PA5-15181), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Preparation of the knockout mice. A: Gene targeting of TNIK locus and RT-PCR of whole brain mRNA from targeted mice. Genomic locus illustrating the region of exons 5-7. Targeting vector indicated showing homology arms, LoxP sites flanking exon 7 (>), and neomycin (G418) resistance cassette used for selection. Neo r cassette is flanked by Frt sites (*). After introduction into the germline, exon 7 was removed by Cre recombinase-mediated deletion. B: RT-PCR of whole brain RNA from animals of the indicated genotypes. PCR primers located in exons 6 and 8 are indicated by arrows. Deletion of exon 7 results in a novel transcript in animals carrying the Delta7 allele that contains early termination codons as indicated. Identity of PCR products was verified by sequencing (data not shown). C,D: Immunofluorescence staining for TNIK in neocortex from WT (C) and TNIK Delta7 /TNIK Delta7 (D) mouse brain. Scale bar = 50 um in C and D.
