PA5-117829

antibody from Invitrogen Antibodies

Targeting: CAMKV MGC8407, VACAMKL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117829

Provider

Invitrogen Antibodies

Product name

CaMKV Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.24 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CaMKV Polyclonal Antibody (Product # PA5-117829) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of CaMKV in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with CaMKV Polyclonal Antibody (Product # PA5-117829, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus and cytoplasm.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CaMKV Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 1 µL with CaMKV Polyclonal Antibody (Product # PA5-117829) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: CaMKV Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CaMKV Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 1 µL with CaMKV Polyclonal Antibody (Product # PA5-117829) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: CaMKV Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa.