AF691

antibody from R&D Systems

Targeting: MST1R CD136, CDw136, PTK8, RON, SEA

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

AF691

Provider

R&D Systems

Product name

Human MSPR/Ron Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Delects human MSP R/Ron in direct ELISAs and Western blots. In these formats, approximately 25% cross-reactivity with recombinant mouse MSP R is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

CAA49634


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Functional consequences of the macrophage stimulating protein 689C inflammatory bowel disease risk allele.

Kauder SE, Santell L, Mai E, Wright LY, Luis E, N'Diaye EN, Lutman J, Ratti N, Sa SM, Maun HR, Stefanich E, Gonzalez LC, Graham RR, Diehl L, Faubion WA Jr, Keir ME, Young J, Chaudhuri A, Lazarus RA, Egen JG
PloS one 2013;8(12):e83958

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Transactivation of RON receptor tyrosine kinase by interaction with PDGF receptor beta during steady-state growth of human mesangial cells.

Kobayashi T, Furukawa Y, Kikuchi J, Ito C, Miyata Y, Muto S, Tanaka A, Kusano E
Kidney international 2009 Jun;75(11):1173-1183

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human MSP R/Ron by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line untreated (-) or treated (+) with 100 uM Pervanadate (PV) for 10 minutes or 100 ng/mL Recombinant Human MSP/MST1 Protein (Catalog # 352-MS) for 10 minutes. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF691) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MSP R/Ron at approximately 150-160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunoprecipitation

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human MSP R/Ron by Immunoprecipitation. Human MSP R/Ron was immunoprecipitated from 1 mg of lysates of MDA-MB-468 human breast cancer cell line untreated (-) or treated (+) with 100 uM Pervanadate (PV) for 10 minutes or 100 ng/mL Recombinant Human MSP/MST1 Protein (Catalog # 352-MS) for 10 minutes. MSP R/Ron was immunoprecipiated with 1 ug Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF691). The MSP R/Ron-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated MSP R/Ron was detected by Western blot using 0.2 µg/mL of Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF691).