Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-82330 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDK12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GFSAIDTDER NSGPALTESL VQTLVKNRTF SGSLSHLGES SSYQGTGSVQ FPGDQDLRFA RVPLALHPVV GQPFLKAEGS SNSVVHAETK LQNYGELGPG TTGASSSGAG LHWGGPTQSS AY
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Cyclin-dependent kinase 12 was achieved by transfecting HeLa cells with Cyclin-dependent kinase 12 specific siRNA (Silencer® select Product # S28623, S28622). Immunofluorescence analysis was performed on untransfected HeLa cells (panel a,d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with Cyclin-dependent kinase 12 specific siRNA (panel c,f). Cells were fixed, permeabilized, and labelled with CDK12 Polyclonal Antibody (Product # PA5-82330, at a concentration of 1 µg/mL) followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300 dilution) was used for cytoskeletal F-actin (Red) staining. Reduction in the specific signal was observed upon siRNA mediated knockdown (panel c,f) confirming specificity of the antibody to Cyclin-dependent kinase 12. The images were captured at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CDK12 in A-431 cells using a CDK12 polyclonal antibody (Product # PA5-82330). The analysis shows localization to nucleoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CDK12 in human pancreas using a CDK12 polyclonal antibody (Product # PA5-82330). The analysis shows strong nuclear positivity in exocrine glandular cells and islet cells.