Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-87011 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-87011, RRID:AB_1199396
- Product name
- Rabbit Polyclonal CrkRS Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Therapeutic Targeting of CDK12/CDK13 in Triple-Negative Breast Cancer.
Cyclin-dependent kinase 12 increases 3' end processing of growth factor-induced c-FOS transcripts.
3' end formation of pre-mRNA and phosphorylation of Ser2 on the RNA polymerase II CTD are reciprocally coupled in human cells.
Quereda V, Bayle S, Vena F, Frydman SM, Monastyrskyi A, Roush WR, Duckett DR
Cancer cell 2019 Nov 11;36(5):545-558.e7
Cancer cell 2019 Nov 11;36(5):545-558.e7
Cyclin-dependent kinase 12 increases 3' end processing of growth factor-induced c-FOS transcripts.
Eifler TT, Shao W, Bartholomeeusen K, Fujinaga K, Jäger S, Johnson JR, Luo Z, Krogan NJ, Peterlin BM
Molecular and cellular biology 2015 Jan;35(2):468-78
Molecular and cellular biology 2015 Jan;35(2):468-78
3' end formation of pre-mRNA and phosphorylation of Ser2 on the RNA polymerase II CTD are reciprocally coupled in human cells.
Davidson L, Muniz L, West S
Genes & development 2014 Feb 15;28(4):342-56
Genes & development 2014 Feb 15;28(4):342-56
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CrkRS Antibody [NB100-87011] - Detection of Human and Mouse CrkRS by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CrkRS antibody NB100-87011 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CrkRS Antibody [NB100-87011] - Detection of Human CrkRS by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CrkRS antibody NB100-87011 used for IP at 3 mcg/mg lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CrkRS Antibody [NB100-87011] - Detection of human CrkRS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CrkRS antibody NB100-87011 used for IP at 6 ug per reaction. For blotting immunoprecipitated CrkRS, NB100-87011 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
