PA5-14462

antibody from Invitrogen Antibodies

Targeting: CDK12 CRK7, CRKR, CRKRS, KIAA0904

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-14462

Provider

Invitrogen Antibodies

Product name

CDK12 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with rat based on sequence homology.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CrkRS using a CrkRS polyclonal antibody (Product # PA5-14462) in mouse liver tissue lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of Cyclin-dependent kinase 12 was achieved by transfecting HeLa cells with Cyclin-dependent kinase 12 specific siRNA (Silencer® select Product # S28623, S28622). Immunofluorescence analysis was performed on untransfected HeLa cells (panel a,d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with Cyclin-dependent kinase 12 specific siRNA (panel c,f). Cells were fixed, permeabilized, and labelled with CDK12 Polyclonal Antibody (Product # PA5-14462, 1:100 dilution) followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300 dilution) was used for cytoskeletal F-actin (Red) staining. Reduction of specific signal was observed upon siRNA mediated knockdown (panel c,f) confirming specificity of the antibody to Cyclin-dependent kinase 12. The Images were captured at 60X magnification.