21066-1-AP

antibody from Proteintech Group

Targeting: MLKL FLJ34389

Provider product page for 21066-1-AP

Western blot

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Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [52]
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Validations
Western blot [1]

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Product number: 21066-1-AP - Provider product page
Provider: Proteintech Group
Product name: MLKL antibody
Antibody type: Polyclonal
Description: KD/KO validated MLKL antibody (Cat. #21066-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

MLKL protein structure - 21066-1-AP shown in red.

Submitted references P. gingivalis alters lung microbiota and aggravates disease severity of COPD rats by up-regulating Hsp90α/MLKL.

Feng N, Han X, Peng D, Geng F, Li Q, Pan C, Wang H, Pan Y, Tan L
Journal of oral microbiology 2024;16(1):2334588

Shikonin and chitosan-silver nanoparticles synergize against triple-negative breast cancer through RIPK3-triggered necroptotic immunogenic cell death.

Liang J, Tian X, Zhou M, Yan F, Fan J, Qin Y, Chen B, Huo X, Yu Z, Tian Y, Deng S, Peng Y, Wang Y, Liu B, Ma X
Biomaterials 2024 Sep;309:122608

Scd1 and monounsaturated lipids are required for autophagy and survival of adipocytes.

Mori H, Peterson SK, Simmermon RC, Overmyer KA, Nishii A, Paulsson E, Li Z, Jen A, Uranga RM, Maung JN, Yacawych WT, Lewis KT, Schill RL, Hetrick T, Seino R, Inoki K, Coon JJ, MacDougald OA
Molecular metabolism 2024 May;83:101916

Nattokinase attenuates endothelial inflammation through the activation of SRF and THBS1.

Chiu HW, Chou CL, Lee KT, Shih CC, Huang TH, Sung LC
International journal of biological macromolecules 2024 May;268(Pt 2):131779

A TBK1 variant causes autophagolysosomal and motoneuron pathology without neuroinflammation in mice.

Brenner D, Sieverding K, Srinidhi J, Zellner S, Secker C, Yilmaz R, Dyckow J, Amr S, Ponomarenko A, Tunaboylu E, Douahem Y, Schlag JS, Rodríguez Martínez L, Kislinger G, Niemann C, Nalbach K, Ruf WP, Uhl J, Hollenbeck J, Schirmer L, Catanese A, Lobsiger CS, Danzer KM, Yilmazer-Hanke D, Münch C, Koch P, Freischmidt A, Fetting M, Behrends C, Parlato R, Weishaupt JH
The Journal of experimental medicine 2024 May 6;221(5)

Electroacupuncture Inhibits Neuroinflammation Induced by Astrocytic Necroptosis Through RIP1/MLKL/TLR4 Pathway in a Mouse Model of Spinal Cord Injury.

Zhao H, Zong X, Li L, Li N, Liu C, Zhang W, Li J, Yang C, Huang S
Molecular neurobiology 2024 Jun;61(6):3258-3271

The anti-cancer drug candidate CBL0137 induced necroptosis via forming left-handed Z-DNA and its binding protein ZBP1 in liver cells.

Li J, Tang M, Ke RX, Li PL, Sheng ZG, Zhu BZ
Toxicology and applied pharmacology 2024 Jan;482:116765

Dissecting cell death pathways in fed-batch bioreactors.

Mentlak DA, Raven J, Moses T, Massie F, Barber N, Hoare R, Burton G, Young A, Pybus LP, Rosser S, White RJ, Ungar D, Bryant NJ
Biotechnology journal 2024 Jan;19(1):e2300257

HIST3H2A promotes the progression of prostate cancer through inhibiting cell necroptosis.

Yang L, Ruan Y, Xu H
BMC cancer 2024 Apr 29;24(1):544

Modulation of YBX1-mediated PANoptosis inhibition by PPM1B and USP10 confers chemoresistance to oxaliplatin in gastric cancer.

Lin C, Lin P, Yao H, Liu S, Lin X, He R, Teng Z, Zuo X, Li Y, Ye J, Zhu G
Cancer letters 2024 Apr 10;587:216712

Physiological ischemic training improves cardiac function through the attenuation of cardiomyocyte apoptosis and the activation of the vagus nerve in chronic heart failure.

Zhu X, Wang S, Cheng Y, Gu H, Zhang X, Teng M, Zhang Y, Wang J, Hua W, Lu X
Frontiers in neuroscience 2023;17:1174455

RNA-sequencing approach for exploring the protective mechanisms of dexmedetomidine on pancreatic injury in severe acute pancreatitis.

Yao J, Lan B, Ma C, Liu Y, Wu X, Feng K, Chen H, Wen Q
Frontiers in pharmacology 2023;14:1189486

N-acetyl-L-cysteine alleviated the oxidative stress-induced inflammation and necroptosis caused by excessive NiCl(2) in primary spleen lymphocytes.

Zhang X, Xu L, Ma W, Shi B, Liu Q, Song Y, Fang C, Liu P, Qiao S, Cai J, Zhang Z
Frontiers in immunology 2023;14:1146645

Whether the Subacute MPTP-Treated Mouse is as Suitable as a Classic Model of Parkinsonism.

Qi Y, Zhang Z, Li Y, Zhao G, Huang J, Zhang Y, Xue J, Tang X
Neuromolecular medicine 2023 Sep;25(3):360-374

SCD1 and monounsaturated lipids are required for autophagy and survival of adipocytes.

Mori H, Peterson SK, Simmermon R, Overmyer KA, Nishii A, Paulsson E, Li Z, Jen A, Uranga RM, Maung J, Yacawych WT, Lewis KT, Schill RL, Hetrick T, Seino R, Inoki K, Coon JJ, MacDougald OA
bioRxiv : the preprint server for biology 2023 Oct 27;

Sulconazole Induces PANoptosis by Triggering Oxidative Stress and Inhibiting Glycolysis to Increase Radiosensitivity in Esophageal Cancer.

Liu LX, Heng JH, Deng DX, Zhao H, Zheng ZY, Liao LD, Lin W, Xu XE, Li EM, Xu LY
Molecular & cellular proteomics : MCP 2023 Jun;22(6):100551

Identification of necroptosis-related gene TRAF5 as potential target of diagnosing atherosclerosis and assessing its stability.

Peng Z, Wang K, Wang S, Wu R, Yao C
BMC medical genomics 2023 Jun 17;16(1):139

Thymol Ameliorates Aspergillus fumigatus Keratitis by Downregulating the TLR4/ MyD88/ NF-kB/ IL-1β Signal Expression and Reducing Necroptosis and Pyroptosis.

Wang L, Yan H, Chen X, Han L, Liu G, Yang H, Lu D, Liu W, Che C
Journal of microbiology and biotechnology 2023 Jan 28;33(1):43-50

MLKL deficiency attenuated hepatocyte oxidative DNA damage by activating mitophagy to suppress macrophage cGAS-STING signaling during liver ischemia and reperfusion injury.

Xu J, Wu D, Zhou S, Hu H, Li F, Guan Z, Zhan X, Gao Y, Wang P, Rao Z
Cell death discovery 2023 Feb 10;9(1):58

Flanking N- and C-terminal domains of PrsA in Streptococcus suis type 2 are crucial for inducing cell death independent of TLR2 recognition.

Jiang X, Yu G, Zhu L, Siddique A, Zhan D, Zhou L, Yue M
Virulence 2023 Dec;14(1):2249779

Blue Light Induces RPE Cell Necroptosis, Which Can Be Inhibited by Minocycline.

Song W, Zhu R, Gao W, Xing C, Yang L
Frontiers in medicine 2022;9:831463

A novel necroptosis-related gene index for predicting prognosis and a cold tumor immune microenvironment in stomach adenocarcinoma.

Khan M, Lin J, Wang B, Chen C, Huang Z, Tian Y, Yuan Y, Bu J
Frontiers in immunology 2022;13:968165

Coeloglossum viride Var. Bracteatum Extract Attenuates MPTP-Induced Neurotoxicity in vivo by Restoring BDNF-TrkB and FGF2-Akt Signaling Axis and Inhibiting RIP1-Driven Inflammation.

Lang XY, Hu Y, Bai JP, Wang J, Qin XY, Lan R
Frontiers in pharmacology 2022;13:903235

Acteoside attenuates RSV-induced lung injury by suppressing necroptosis and regulating metabolism.

Ling X, Zhou J, Jin T, Xu W, Sun X, Li W, Ding Y, Liang M, Zhu C, Zhao P, Hu C, Yuan B, Xie T, Tao J
Frontiers in pharmacology 2022;13:870928

Mesenchymal Stem Cells-Derived Exosomes Ameliorate Ischemia/Reperfusion Induced Acute Kidney Injury in a Porcine Model.

Huang J, Cao H, Cui B, Ma X, Gao L, Yu C, Shen F, Yang X, Liu N, Qiu A, Cai G, Zhuang S
Frontiers in cell and developmental biology 2022;10:899869

Polymyxin B Reduces Brain Injury in Ischemic Stroke Rat Through a Mechanism Involving Targeting ESCRT-III Machinery and RIPK1/RIPK3/MLKL Pathway.

Tian J, Zhang YY, Peng YW, Liu B, Zhang XJ, Hu ZY, Hu CP, Luo XJ, Peng J
Journal of cardiovascular translational research 2022 Oct;15(5):1129-1142

Tea polyphenols alleviates acetochlor-induced apoptosis and necroptosis via ROS/MAPK/NF-κB signaling in Ctenopharyngodon idellus kidney cells.

Zhao X, Shi X, Liu Q, Li X
Aquatic toxicology (Amsterdam, Netherlands) 2022 May;246:106153

Salidroside alleviates cadmium-induced toxicity in mice by restoring the notch/HES-1 and RIP1-driven inflammatory signaling axis.

Shu XM, Hu Y, Fang X, Wang J, Qin XY, Lan R
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2022 Jun;71(5-6):615-626

Selenium Deficiency Induces Apoptosis and Necroptosis Through ROS/MAPK Signal in Human Uterine Smooth Muscle Cells.

Wang Y, Li X, Yao Y, Zhao X, Shi X, Cai Y
Biological trace element research 2022 Jul;200(7):3147-3158

Mixed lineage kinase domain-like pseudokinase-mediated necroptosis aggravates periodontitis progression.

Yang Y, Wang L, Zhang H, Luo L
Journal of molecular medicine (Berlin, Germany) 2022 Jan;100(1):77-86

Role of HMGB1 in TNF-α Combined with Z-VAD-fmk-Induced L929 Cells Necroptosis.

Yu C, Lei Z, Li X, Huang LH, Li ZQ, Zhu HW, Han D, Huang H, Yu X
Biochemical genetics 2022 Apr;60(2):598-610

Pterostilbene attenuates RIPK3-dependent hepatocyte necroptosis in alcoholic liver disease via SIRT2-mediated NFATc4 deacetylation.

Shao Y, Wang X, Zhou Y, Jiang Y, Wu R, Lu C
Toxicology 2021 Sep;461:152923

Pterocephin A, a novel Triterpenoid Saponin from Pterocephalus hookeri induced liver injury by activation of necroptosis.

Wang R, Wei L, Dong Z, Meng F, Wang G, Zhou S, Lan X, Liao Z, Chen M
Phytomedicine : international journal of phytotherapy and phytopharmacology 2021 May;85:153548

Toxoplasma gondii secreted effectors co-opt host repressor complexes to inhibit necroptosis.

Rosenberg A, Sibley LD
Cell host & microbe 2021 Jul 14;29(7):1186-1198.e8

STAT3β Enhances Sensitivity to Concurrent Chemoradiotherapy by Inducing Cellular Necroptosis in Esophageal Squamous Cell Carcinoma.

Zheng ZY, Yang PL, Luo W, Yu SX, Xu HY, Huang Y, Li RY, Chen Y, Xu XE, Liao LD, Wang SH, Huang HC, Li EM, Xu LY
Cancers 2021 Feb 21;13(4)

Self-assembled polymeric nanocarrier-mediated co-delivery of metformin and doxorubicin for melanoma therapy.

Song M, Xia W, Tao Z, Zhu B, Zhang W, Liu C, Chen S
Drug delivery 2021 Dec;28(1):594-606

Traditional herbal formula Wu-Mei-Wan alleviates TNBS-induced colitis in mice by inhibiting necroptosis through increasing RIPK3 O-GlcNAcylation.

Wu F, Shao Q, Cheng Z, Xiong X, Fang K, Zhao Y, Dong R, Xu L, Lu F, Chen G
Chinese medicine 2021 Aug 16;16(1):78

Cadmium exposure induces TNF-α-mediated necroptosis via FPR2/TGF-β/NF-κB pathway in swine myocardium.

Zhao X, Wang S, Li X, Liu H, Xu S
Toxicology 2021 Apr 15;453:152733

RIP1/RIP3/MLKL mediates dopaminergic neuron necroptosis in a mouse model of Parkinson disease.

Lin QS, Chen P, Wang WX, Lin CC, Zhou Y, Yu LH, Lin YX, Xu YF, Kang DZ
Laboratory investigation; a journal of technical methods and pathology 2020 Mar;100(3):503-511

New insights in acetaminophen toxicity: HMGB1 contributes by itself to amplify hepatocyte necrosis in vitro through the TLR4-TRIF-RIPK3 axis.

Minsart C, Liefferinckx C, Lemmers A, Dressen C, Quertinmont E, Leclercq I, Devière J, Moreau R, Gustot T
Scientific reports 2020 Mar 27;10(1):5557

Norfloxacin induces apoptosis and necroptosis in human corneal epithelial cells.

Yang T, Fan TJ, Xu B
Toxicology in vitro : an international journal published in association with BIBRA 2020 Aug;66:104868

A necroptotic-independent function of MLKL in regulating endothelial cell adhesion molecule expression.

Dai J, Zhang C, Guo L, He H, Jiang K, Huang Y, Zhang X, Zhang H, Wei W, Zhang Y, Lu L, Hu J
Cell death & disease 2020 Apr 24;11(4):282

Quetiapine induces myocardial necroptotic cell death through bidirectional regulation of cannabinoid receptors.

Li X, Peng Z, Zhou Y, Wang J, Lin X, Dong X, Liu X, Jiang J, Jiang Y, Li L
Toxicology letters 2019 Oct 1;313:77-90

FKBP12 mediates necroptosis by initiating RIPK1-RIPK3-MLKL signal transduction in response to TNF receptor 1 ligation.

Wang Z, Feng J, Yu J, Chen G
Journal of cell science 2019 May 20;132(10)

Dexmedetomidine Preconditioning Protects Cardiomyocytes Against Hypoxia/Reoxygenation-Induced Necroptosis by Inhibiting HMGB1-Mediated Inflammation.

Chen J, Jiang Z, Zhou X, Sun X, Cao J, Liu Y, Wang X
Cardiovascular drugs and therapy 2019 Feb;33(1):45-54

Visfatin Plays a Significant Role in Alleviating Lipopolysaccharide-Induced Apoptosis and Autophagy Through PI3K/AKT Signaling Pathway During Acute Lung Injury in Mice.

Wu XT, Ansari AR, Pang XX, Li HZ, Zhang ZW, Luo Y, Arshad M, Song H
Archivum immunologiae et therapiae experimentalis 2019 Aug;67(4):249-261

Neferine, a Bisbenzylisoquinoline Alkaloid, Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis.

Wu X, Guo Y, Min X, Pei L, Chen X
The American journal of Chinese medicine 2018;46(6):1263-1279

Necrostatin-1 promotes ectopic periodontal tissue like structure regeneration in LPS-treated PDLSCs.

Yan B, Zhang H, Dai T, Gu Y, Qiu X, Hu C, Liu Y, Wei K, Li D
PloS one 2018;13(11):e0207760

Receptor-Interacting Protein 3/Caspase-8 May Regulate Inflammatory Response and Promote Tissue Regeneration in the Periodontal Microenvironment.

Yan B, Wei K, Hou L, Dai T, Gu Y, Qiu X, Chen J, Feng Y, Cheng H, Yu Z, Zhang Y, Zhang H, Li D
Medical science monitor : international medical journal of experimental and clinical research 2018 Jul 29;24:5247-5257

Dihydrotanshinone I, a natural product, ameliorates DSS-induced experimental ulcerative colitis in mice.

Guo Y, Wu X, Wu Q, Lu Y, Shi J, Chen X
Toxicology and applied pharmacology 2018 Apr 1;344:35-45

Neuroprotective Effect of β-Caryophyllene on Cerebral Ischemia-Reperfusion Injury via Regulation of Necroptotic Neuronal Death and Inflammation: In Vivo and in Vitro.

Yang M, Lv Y, Tian X, Lou J, An R, Zhang Q, Li M, Xu L, Dong Z
Frontiers in neuroscience 2017;11:583

A novel sgRNA selection system for CRISPR-Cas9 in mammalian cells.

Zhang H, Zhang X, Fan C, Xie Q, Xu C, Zhao Q, Liu Y, Wu X, Zhang H
Biochemical and biophysical research communications 2016 Mar 18;471(4):528-32

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 21066-1-AP( MLKL Antibody) at dilution of 1:300
Other comments: WB
Appendix: 21066-1-AP.pdf

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