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- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
- Other assay [2]
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- Product number
- CF801287 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALK Monoclonal Antibody (OTI1A4), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI1A4
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALK (RC222485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ALK. Positive lysates LY418072 (100 µg) and LC418072 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALK (RC222485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ALK. Positive lysates LY418072 (100 µg) and LC418072 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35 µg) from H2228 and A549 cell lines by using anti-ALK monoclonal antibody. (TA801287, 1:10,000)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of paraffin-embedded human cell line H1975 (upper left) and H2228 (upper right) on IHC antibody quality control slide using anti-ALK mouse monoclonal antibody TA801287 (1:400). The ALK rearrangement in H2228 cells is labeled with ALK Breakapart probe in FISHtest (lower right, 60X) and the control of H1975 cell at the same FISHprobe test (lower left, 60X).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human ALK-positive lung cancer tissue using anti-ALK mouse monoclonal antibody. (TA801287, 1:100 for 30 min at RT; heat-induced epitope retrieval by TEE, pH9.0)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure from citation: Immunohistochemistry of protein level by using anti-ALK antibody in human right cervical lymph node. Dilution: 1:200 View Citation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human non-small cell lung cancer sample with ALK translocation detected by FISH using anti-ALK mouse monoclonal antibody. (TA801287, 1:50; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human large B cell lymphoma with ALK translocation using anti-ALK mouse monoclonal antibody. (TA801287, 1:50; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded ALK-positive lung tumor xenograft using anti-ALK mouse monoclonal antibody. (TA801287, 1:50; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human non-small cell lung cancer sample with EML4-ALK translocation detected by PCR using anti-ALK mouse monoclonal antibody. (TA801287, 1:50; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ALK Luminex with 15B2 Capture (TA801288) and 1A4 Detection (TA801287) Antibodies. Substrate used: full length HEK293 cells expressed recombinant ALK protein (TP322485).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ALK Luminex with 10D7 Capture (TA801306) and 1A4 Detection (TA801287) Antibodies. Substrate used: full length HEK293 cells expressed recombinant ALK protein (TP322485).
