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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00009943-M14 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00009943-M14, RRID:AB_581743
- Product name
- OXSR1 monoclonal antibody (M14), clone 1B10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant OXSR1.
- Antigen sequence
MSEDSSALPWSINRDDYELQEVIGSGATAVVQAAY
CAPKKEKVAIKRINLEKCQTSMDELLKEIQAMSQC
HHPNIVSYYTSFVVKDELWLVMKLLSGGSVLDIIK
HIVAKGEHKSGVLDESTIATILREVLEGLEYLHKN
GQIHRDVKAGNILLGEDGSVQIADFGVSAFLATGG
DITRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADI
WSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPP
SLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRP
TAAELLRHKFFQKAKNKEFLQEKTLQRAPTISERA
KKVRRVPGSSGRLHKTEDGGWEWSDDEFDEESEEG
KAAISQLRSPRVKESISNSELFPTTDPVGTLLQVP
EQISAHLPQPAGQIATQPTQVSLPPTAEPAKTAQA
LSSGSGSQETKIPISLVLRLRNSKKELNDIRFEFT
PGRDTAEGVSQELISAGLVDGRDLVIVAANLQKIV
EEPQSNRSVTFKLASGVEGSDIPDDGKLIGFAQLS
IS- Isotype
- IgG
- Antibody clone number
- 1B10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of OXSR1 expression in transfected 293T cell line by OXSR1 monoclonal antibody (M14), clone 1B10.Lane 1: OXSR1 transfected lysate (Predicted MW: 58 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged OXSR1 is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of OXSR1 transfected lysate using anti-OXSR1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with OXSR1 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
