PA5-21989

antibody from Invitrogen Antibodies

Targeting: TNNI3K CARK

Provider product page for PA5-21989

Western blot

Immunocytochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [1]
Comments [0]
Validations
Immunocytochemistry [2]
Other assay [3]

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Product number: PA5-21989 - Provider product page
Provider: Invitrogen Antibodies
Product name: TNNI3K Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Raji. Predicted reactivity: Mouse (93%), Rat (93%), Zebrafish (83%), Dog (97%), Rabbit (97%), Chicken (90%), Rhesus Monkey (97%), Chimpanzee (99%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

TNNI3K protein structure - PA5-21989 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 2 The Tnni3k gene in naked mole-rats ( H . glaber ). A. Genomic DNA sequence at the end of intron 15 and beginning of exon 16 and deduced protein coding sequence (in 1 letter code above) in mouse (Mmu) and in H . glaber (Hgla). The in-frame premature stop codon is indicated in red type, and lies at position 22288559-561 (reverse complement) of NW_004624742.1 and at 1144837-839 (reverse complement) of NW_004629930.1. The sequence trace confirms the accuracy of the H . glaber genomic sequence. B. Western blot to detect Tnni3k; the blot contains ventricular protein from one wild-type (WT) C57BL/6 mouse, one homozygous (-/-) mouse, and two individual H . glaber animals. C. Ventricular mononuclear CM% in adult hearts of two inbred mouse strains (SJL/J and A/J), which represent the lowest and highest known values of mononuclear CM% among common inbred strains, and in adult H . glaber . Data for SJL/J and A/J mice are from our previous evaluation [ 20 ] and are shown here for reference. D. Evaluation of nuclear ploidy in single cell preparations from one C57BL/6J mouse (at left) and four naked mole-rats, as measured by DAPI fluorescence. Values shown represent fluorescence intensity per nucleus from mono- and bi-nucleated cardiomyocytes and endothelial cells, normalized to the median intensity of endothelial cell nuclei; a normalized value of 1.0 is assumed to be the median signal from a diploid nucleus. Each dot is one measured nucleus. Numerical quantitations of 2N and >=

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 3 Consequences of a kinase-dead Tnni3k allele in mice. A. Western blot of Tnni3k protein from mice of the indicated genotypes, demonstrating that the K489R protein is stable in vivo. B. Ventricular mononuclear CM% in mice of the indicated Tnni3k genotypes; heterozygous (+/-) and (-/-) mice were littermates of the K489R/- mice, but not of each other. C. Evaluation of nuclear ploidy specifically in the mononuclear CM subpopulation of mice of the indicated Tnni3k genotypes. See also S4 Fig .

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 5 Tnni3k converges with oxidative stress. A. Western blot of ventricular protein from two wild-type and two Tnni3k P3 mice showing phosphorylated (activated) and total protein for the three MAPK members p38, Jnk, and Erk. The very low level of phospho-p38 in P3 heart is compared against the normal level in adult heart in S6 Fig . B-C. Ventricular mononuclear CM% in adult hearts, comparing absence vs. presence of the mCAT transgene in a background that is wild-type for Tnni3k and heterozygous for Nnt (B), and comparing absence vs. presence of the mCAT transgene in a Tnni3k and Nnt background (C).