Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AF432 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal EphB3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse EphB3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human EphB3 is observed and less than 1% cross-reactivity with recombinant rat EphB1, recombinant mouse (rm) EphA4, rmEphB2, rmEphB4, and rmEphB6 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references CD74 Signaling Links Inflammation to Intestinal Epithelial Cell Regeneration and Promotes Mucosal Healing.
Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation.
Tissue-Specific Effects of Reduced β-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium.
Distinct fibroblast lineages determine dermal architecture in skin development and repair.
Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression.
Ephrin-B1 regulates axon guidance by reverse signaling through a PDZ-dependent mechanism.
The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.
EphB receptors coordinate migration and proliferation in the intestinal stem cell niche.
EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases.
Farr L, Ghosh S, Jiang N, Watanabe K, Parlak M, Bucala R, Moonah S
Cellular and molecular gastroenterology and hepatology 2020;10(1):101-112
Cellular and molecular gastroenterology and hepatology 2020;10(1):101-112
Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation.
O'Neill AK, Kindberg AA, Niethamer TK, Larson AR, Ho HH, Greenberg ME, Bush JO
The Journal of cell biology 2016 Oct 24;215(2):217-229
The Journal of cell biology 2016 Oct 24;215(2):217-229
Tissue-Specific Effects of Reduced β-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium.
Feng Y, Sakamoto N, Wu R, Liu JY, Wiese A, Green ME, Green M, Akyol A, Roy BC, Zhai Y, Cho KR, Fearon ER
PLoS genetics 2015 Nov;11(11):e1005638
PLoS genetics 2015 Nov;11(11):e1005638
Distinct fibroblast lineages determine dermal architecture in skin development and repair.
Driskell RR, Lichtenberger BM, Hoste E, Kretzschmar K, Simons BD, Charalambous M, Ferron SR, Herault Y, Pavlovic G, Ferguson-Smith AC, Watt FM
Nature 2013 Dec 12;504(7479):277-281
Nature 2013 Dec 12;504(7479):277-281
Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression.
Furukawa K, Sato T, Katsuno T, Nakagawa T, Noguchi Y, Tokumasa A, Yokote K, Yokosuka O, Saito Y
Biochemical and biophysical research communications 2011 Feb 25;405(4):521-6
Biochemical and biophysical research communications 2011 Feb 25;405(4):521-6
Ephrin-B1 regulates axon guidance by reverse signaling through a PDZ-dependent mechanism.
Bush JO, Soriano P
Genes & development 2009 Jul 1;23(13):1586-99
Genes & development 2009 Jul 1;23(13):1586-99
The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.
Yang NY, Pasquale EB, Owen LB, Ethell IM
The Journal of biological chemistry 2006 Oct 27;281(43):32574-86
The Journal of biological chemistry 2006 Oct 27;281(43):32574-86
EphB receptors coordinate migration and proliferation in the intestinal stem cell niche.
Holmberg J, Genander M, Halford MM, Annerén C, Sondell M, Chumley MJ, Silvany RE, Henkemeyer M, Frisén J
Cell 2006 Jun 16;125(6):1151-63
Cell 2006 Jun 16;125(6):1151-63
EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases.
Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S
Journal of cell science 2006 Feb 1;119(Pt 3):559-70
Journal of cell science 2006 Feb 1;119(Pt 3):559-70
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse EphB3 by Western Blot. Detection of Mouse EphB3 by Western Blot.