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Western blot
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- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00002049-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002049-M01, RRID:AB_538603
- Product name
- Mouse Monoclonal EphB3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. EPHB3 - EPH receptor B3
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references EphB3 signaling induces cortical endothelial cell death and disrupts the blood-brain barrier after traumatic brain injury.
The simultaneous expression of both ephrin B3 receptor and E-cadherin in Barrett`s adenocarcinoma is associated with favorable clinical staging.
Complementary expression of EphB receptors and ephrin-B ligand in the pyloric and duodenal epithelium of adult mice.
Class I and III HDACs and loss of active chromatin features contribute to epigenetic silencing of CDX1 and EPHB tumor suppressor genes in colorectal cancer.
Class I and III HDACs and loss of active chromatin features contribute to epigenetic silencing of CDX1 and EPHB tumor suppressor genes in colorectal cancer.
Complementary expression and repulsive signaling suggest that EphB receptors and ephrin-B ligands control cell positioning in the gastric epithelium.
Microarray-based response prediction in esophageal adenocarcinoma.
Assis-Nascimento P, Tsenkina Y, Liebl DJ
Cell death & disease 2018 Jan 8;9(1):7
Cell death & disease 2018 Jan 8;9(1):7
The simultaneous expression of both ephrin B3 receptor and E-cadherin in Barrett`s adenocarcinoma is associated with favorable clinical staging.
Schauer MC, Stoecklein NH, Theisen J, Kröpil F, Baldus S, Hoelscher A, Feith M, Bölke E, Matuschek C, Budach W, Knoefel WT
European journal of medical research 2012 May 14;17:10
European journal of medical research 2012 May 14;17:10
Complementary expression of EphB receptors and ephrin-B ligand in the pyloric and duodenal epithelium of adult mice.
Ishii M, Nakajima T, Ogawa K
Histochemistry and cell biology 2011 Sep;136(3):345-56
Histochemistry and cell biology 2011 Sep;136(3):345-56
Class I and III HDACs and loss of active chromatin features contribute to epigenetic silencing of CDX1 and EPHB tumor suppressor genes in colorectal cancer.
Rönsch K, Jäger M, Schöpflin A, Danciu M, Lassmann S, Hecht A
Epigenetics 2011 May;6(5):610-22
Epigenetics 2011 May;6(5):610-22
Class I and III HDACs and loss of active chromatin features contribute to epigenetic silencing of CDX1 and EPHB tumor suppressor genes in colorectal cancer.
Rönsch K, Jäger M, Schöpflin A, Danciu M, Lassmann S, Hecht A
Epigenetics 2011 May;6(5):610-22
Epigenetics 2011 May;6(5):610-22
Complementary expression and repulsive signaling suggest that EphB receptors and ephrin-B ligands control cell positioning in the gastric epithelium.
Ogawa K, Takemoto N, Ishii M, Pasquale EB, Nakajima T
Histochemistry and cell biology 2011 Dec;136(6):617-36
Histochemistry and cell biology 2011 Dec;136(6):617-36
Microarray-based response prediction in esophageal adenocarcinoma.
Schauer M, Janssen KP, Rimkus C, Raggi M, Feith M, Friess H, Theisen J
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Jan 1;16(1):330-7
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Jan 1;16(1):330-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EphB3 Antibody (1B3) [H00002049-M01] - Analysis of EPHB3 expression in transfected 293T cell line by EPHB3 monoclonal antibody (M01), clone 1B3.Lane 1: EPHB3 transfected lysate(110.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EphB3 Antibody (1B3) [H00002049-M01] - EPHB3 monoclonal antibody (M01), clone 1B3 Analysis of EPHB3 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: EphB3 Antibody (1B3) [H00002049-M01] - Detection limit for recombinant GST tagged EPHB3 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EphB3 Antibody (1B3) [H00002049-M01] - Analysis of monoclonal antibody to EPHB3 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
