Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- HPA008184 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA008184, RRID:AB_1848206
- Product name
- Anti-EPHB3
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human EPHB3, Gene description: EPH receptor B3, Alternative Gene Names: ETK2, Hek2, Tyro6, Validated applications: IHC, WB, Uniprot ID: P54753, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Proteomic profiling of tumour tissue‐derived extracellular vesicles in colon cancer
Cvjetkovic A, Karimi N, Crescitelli R, Thorsell A, Taflin H, Lässer C, Lötvall J
Journal of Extracellular Biology 2024;3(2)
Journal of Extracellular Biology 2024;3(2)
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and EPHB3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401410).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human skin and skeletal muscle tissues using HPA008184 antibody. Corresponding EPHB3 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol