HPA008184

antibody from Atlas Antibodies

Targeting: EPHB3 ETK2, Hek2, Tyro6

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA008184

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA008184, RRID:AB_1848206

Product name

Anti-EPHB3

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

GDGEWMVPVGACTCATGHEPAAKESQCRPCPPGSY
KAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRA
DSDSADSACTTVPSPPRGVISNVNETSLILEWSEP
RDLGGRDDLLYNVICKKCHGAGGASACSRCDDNVE
FVP


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and EPHB3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401410).

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human skin and skeletal muscle tissues using Anti-EPHB3 antibody. Corresponding EPHB3 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skin shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows low expression as expected.

Sample type

HUMAN