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LearnA01708-3
antibody from Boster Biological Technology
Targeting: PRKG1
PGK, PKG, PKG1, PRKG1B, PRKGR1B
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- A01708-3 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-cGKI/PRKG1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for cGKI/PRKG1 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Diet Supplementation of Luteolin before Fatty Liver Formation Improves Hepatic Steatosis in Obese Mice by Inhibiting Visceral Adipose Tissue Lipolysis.
Wang J, Zhang Z, Lin Y, Chen J, Wang F, Wang L, Liu J, Zhang X
Molecular nutrition & food research 2023 Apr;67(7):e2200478
Molecular nutrition & food research 2023 Apr;67(7):e2200478
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of CGKI/PRKG1 using anti-CGKI/PRKG1 antibody (A01708-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CGKI/PRKG1 antigen affinity purified polyclonal antibody (Catalog # A01708-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CGKI/PRKG1 at approximately 78KD. The expected band size for CGKI/PRKG1 is at 78KD.
- Additional image
