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LearnPA1-46194
antibody from Invitrogen Antibodies
Targeting: LRRK2
DKFZp434H2111, FLJ45829, PARK8, RIPK7, ROCO2
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA1-46194 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LRRK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: human lymphoblasts for IHC, human and mouse brain for Western blot.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout validation by Western blot analysis of LRRK2 in lysates of HeLa human cervical epithelial carcinoma parental cell line and LRRK2 knockout (KO) HeLa cell line. Samples were incubated in LRRK2 polyclonal antibody (Product # PA1-46194) using a dilution of 1:1000 followed by a HRP-conjugated Anti-Rabbit IgG secondary antibody. Specific band was detected for LRRK2 at approximately 275 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LRRK2 in human lymphoblast. Sample was incubated in LRRK2 polyclonal antibody (Product # PA1-46194).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of LRRK2 in (A) Substantia nigra neurons (B) Chains of migrating neuroblasts. Samples were incubated in LRRK2 polyclonal antibody (Product # PA1-46194).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of LRRK2 in (A) Substantia nigra neurons (B) Chains of migrating neuroblasts. Samples were incubated in LRRK2 polyclonal antibody (Product # PA1-46194).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of LRRK2 in frozen sections of mouse brainstem. Frozen samples were incubated in LRRK2 polyclonal antibody (Product # PA1-46194).
