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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- PA5-38570 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Histone 1F0 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on acid extracts (20 µg lysate) of PC-12 (Lane 1), PC-12 treated with Retinoic acid (100 uM for 12 days) (Lane 2), HEK-293 (Lane 3), MDA-MB-231 (Lane 4) and DU-145 (Lane 5). The blot was probed with Anti-Histone 1F0 Polyclonal Antibody (Product # PA5-38570, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 30 kDa band corresponding to Histone 1F0 was observed across the cell lines tested and enhanced upon differentiation with retinoic acid.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Enrichment of endogenous Histone H2B protein at specific gene loci using Anti-Histone H2B Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Histone H2B Polyclonal Antibody (Product # PA1-41058, 3 µg) on sheared chromatin from 2 million U-2 OS cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over the GAPDH gene (active) and MYOD, SAT2 satellite repeats, SAT Alpha (inactive). A schematic diagram of the GAPDH gene is shown on top of the figure. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
