LS-C673654

antibody from LSBio

Targeting: H1-0 H1.0, H1F0, H1FV

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C673654

Provider

LSBio

Product name

H1F0 Antibody LS-C673654

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: H1F0 antibody at 1:50 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Positive Western Blot detected in HepG2 whole cell lysate, Rat spleen tissue. All lanes: H1F0 antibody at 1.9 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 21, 20 KDa. Observed band size: 21 KDa

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Positive Western Blot detected in HepG2 whole cell lysate, Rat spleen tissue. All lanes: H1F0 antibody at 1.9 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 21, 20 KDa. Observed band size: 21 KDa

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunocytochemistry analysis of Mono-methyl-H1F0 (K101) Antibody diluted at 1:5 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunocytochemistry analysis of Mono-methyl-H1F0 (K101) Antibody diluted at 1:5 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.