Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- A08821-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Histone H1.0/H1F0 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Histone H1.0/H1F0 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Role of endoplasmic reticulum stress in apoptosis of differentiated mouse podocytes induced by high glucose.
Cao Y, Hao Y, Li H, Liu Q, Gao F, Liu W, Duan H
International journal of molecular medicine 2014 Apr;33(4):809-16
International journal of molecular medicine 2014 Apr;33(4):809-16
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of H1F0 using anti-H1F0 antibody (A08821-1). H1F0 was detected in paraffin-embedded section of mouse small intestine tissues . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-H1F0 Antibody (A08821-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image