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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- V3735 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant Histone H1 Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific Recombinant Histone H1 antibody is suitable for use in Flow cytometry/Immunofluorescence/Immunohistochemistry/Western blot applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- r1415-1
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the recombinant Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human heart lysate with recombinant Histone H1 (clone R1415-1). Observed molecular weight ~22/30-33 kDa (unmodified/modified).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PFA-fixed human HeLa cells with recombinant Histone H1 antibody (clone r1415-1, green) and Phalloidin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil with recombinant Histone H1 antibody (clone r1415-1). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE rat pancreas with recombinant Histone H1 antibody (clone r1415-1). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of permeabilzed human HeLa cells with recombinant Histone H1 antibody (clone r1415-1); Red=isotype control, Blue= recombinant Histone H1 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free recombinant Histone H1 antibody (clone r1415-1) as confirmation of integrity and purity.
