Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-75208 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KSR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The stability of R-spine defines RAF inhibitor resistance: A comprehensive analysis of oncogenic BRAF mutants with in-frame insertion of αC-β4 loop.
Yap J, Deepak RNVK, Tian Z, Ng WH, Goh KC, Foo A, Tee ZH, Mohanam MP, Sim YRM, Degirmenci U, Lam P, Chen Z, Fan H, Hu J
Science advances 2021 Jun;7(24)
Science advances 2021 Jun;7(24)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KSR1 in Lane 1: SGC7901 whole cell lysate (40 µg), Lane 2: HeLa whole cell lysate (40 µg), Lane 3: BV2 whole cell lysate (40 µg), Lane 4: C6 whole cell lysate (40 µg). Samples were incubated with KSR1 polyclonal antibody (Product # PA5-75208) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KSR1 in Lane 1: U-87MG whole cell lysate (40 µg), Lane 2: C6 whole cell lysate (40 µg), Lane 3: the Brain tissue lysate of mouse (40 µg). Samples were incubated with KSR1 polyclonal antibody (Product # PA5-75208) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KSR1 in Lane 1: SGC7901 whole cell lysate (40 µg), Lane 2: HeLa whole cell lysate (40 µg), Lane 3: BV2 whole cell lysate (40 µg), Lane 4: C6 whole cell lysate (40 µg). Samples were incubated with KSR1 polyclonal antibody (Product # PA5-75208) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KSR1 in paraffin-embedded human breast carcinoma tissue. Samples were incubated with KSR1 polyclonal antibody (Product # PA5-75208) at a dilution of 1:50.