Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 712043 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- JIK Recombinant Polyclonal Antibody (1HCLC)
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- This antibody is predicted to react with Monkey, Pig, Bat.
- Antibody clone number
- 1HCLC
- Concentration
- 0.5 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of Raji (Lane 1) and Jurkat (Lane 2). The blot was probed with Anti-JIK Recombinant Rabbit Polyclonal Antibody (Product # 712043, 1:330 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). A ~115 kDa band corresponding to JIK was observed in the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis, LNCaP cells were fixed and permeabilized for detection of endogenous JIK using Anti-JIK Recombinant Rabbit Polyclonal Antibody (Product # 712043, 1:100) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Panel a-d) clearly demonstrates membrane localization of JIK in LNCaP cells. Panel e-h) shows no specific staining in DU145 cells which is JIK negative, demonstrating specificity. The images were captured at 60X magnification.
