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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-15214 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP26B1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Cardiac retinoic acid levels decline in heart failure.
Yang N, Parker LE, Yu J, Jones JW, Liu T, Papanicolaou KN, Talbot CC Jr, Margulies KB, O'Rourke B, Kane MA, Foster DB
JCI insight 2021 Apr 22;6(8)
JCI insight 2021 Apr 22;6(8)
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 6 Remodeling of the enzymes of ATRA metabolism differs between guinea pig and human heart failure. ( A ) Guinea pig HF: Groups are as defined in Figure 3 . N values are given in parentheses. ALDH1A1 protein levels followed a trend of progressive downregulation in HF that differed significantly from Ctrl at 2 weeks and 4 weeks. LIMMA-moderated t test as in ref. ; SS : P < 0.001, Y= : P < 0.01. Transcript levels of CYP26A1 fell significantly by 4-week HF, whereas levels of CYP26B1 and CYP26C1 were unchanged (1-way ANOVA, post hoc Tukey''s HSD; *: P < 0.05). ( B ) Human IDCM: immunoblot signal intensities were analyzed as detailed in Methods. Group mean differences were assessed by a 2-tailed t test ( # : P < 0.0001). Immunoblots indicate that retinaldehyde dehydrogenase 1 (ALDH1A1) was expressed at low levels in the myocardium of healthy donors but was increased approximately 10-fold in patients with IDCM. CYP26A1 levels were highly variable and substantially upregulated in IDCM. CYP26B1 was only detected marginally and not quantified. CYP26C1 was detected in human hearts, but levels did not differ significantly between donors and patients.
