Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-89910 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC7A2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: PRNLSSPFIF HEKTSEF Positive Samples: 293T, HepG2, HeLa, Mouse liver, Mouse brain, Rat liver; Cellular Location: Cell membrane, Multi-pass membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.56 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using SLC7A2 Polyclonal antibody (Product # PA5-89910) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SLC7A2 in extracts of various cell lines using SLC7A2 Polyclonal Antibody (Product # PA5-89910) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SLC7A2 in paraffin-embedded human mammary cancer using SLC7A2 Polyclonal Antibody (Product # PA5-89910) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SLC7A2 in paraffin-embedded human placenta using SLC7A2 Polyclonal Antibody (Product # PA5-89910) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SLC7A2 in paraffin-embedded rat ovary using SLC7A2 Polyclonal Antibody (Product # PA5-89910) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SLC7A2 in paraffin-embedded mouse testis using SLC7A2 Polyclonal Antibody (Product # PA5-89910) at a dilution of 1:100.