Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 13-9018-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD266 (TWEAK Receptor) Monoclonal Antibody (ITEM-4), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. It has been reported that ITEM-4 cross-reacts with mouse Fn14. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line. The ITEM-4 antibody has been show to have blocking function. Applications Reported: The ITEM-4 antibody has been reported for use in flow cytometric analysis. Applications Tested: The ITEM-4 antibody has been tested by flow cytometric analysis on human and mouse Fn14 transfected cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- ITEM-4
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, do not freeze
Submitted references Soluble and transmembrane TNF-like weak inducer of apoptosis differentially activate the classical and noncanonical NF-kappa B pathway.
The CD163-expressing macrophages recognize and internalize TWEAK: potential consequences in atherosclerosis.
Roos C, Wicovsky A, Müller N, Salzmann S, Rosenthal T, Kalthoff H, Trauzold A, Seher A, Henkler F, Kneitz C, Wajant H
Journal of immunology (Baltimore, Md. : 1950) 2010 Aug 1;185(3):1593-605
Journal of immunology (Baltimore, Md. : 1950) 2010 Aug 1;185(3):1593-605
The CD163-expressing macrophages recognize and internalize TWEAK: potential consequences in atherosclerosis.
Moreno JA, Muñoz-García B, Martín-Ventura JL, Madrigal-Matute J, Orbe J, Páramo JA, Ortega L, Egido J, Blanco-Colio LM
Atherosclerosis 2009 Nov;207(1):103-10
Atherosclerosis 2009 Nov;207(1):103-10
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of non-transfected (left) and human CD266 transfected (right) L5178Y cells with 0.25 µg of Mouse IgG2b Iso Control (Product # 13-4732-85) (open histogram) or 0.25 µg Anti-Human/Mouse CD266 (TWEAK Receptor) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were analyzed.
- Conjugate
- Biotin