LS-C290909

antibody from LSBio

Targeting: TRIO ARHGEF23

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

LS-C290909

Provider

LSBio

Product name

Anti-TRIO Antibody (aa1575-1625) LS-C290909

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Vial size

100µl

Storage

Store at 2-8°C for up to 1 year.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human TRIO by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TRIO antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human TRIO by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-TRIO antibody used for IP at 6 ug per reaction. TRIO was also immunoprecipitated by rabbit anti-TRIO antibody BL15749. For blotting immunoprecipitated TRIO, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.