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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-78532 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCAR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: 293T, A431, HeLa, HepG2 Predicted Reactivity: Rat (97%), Xenopus laevis (82%), Xenopus Tropicalis (84%), Rhesus Monkey (100%), Bovine (99%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CCAR1 in A) 293T whole cell lysate, B) A431 whole cell lysate, C) HeLa whole cell lysate, D) HepG2 whole cell lysate using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with CCAR1 polyclonal antibody (Product # PA5-78532) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CCAR1 Polyclonal Antibody detects CCAR1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract.5 % SDS-PAGE. CCAR1 Polyclonal Antibody (Product # PA5-78532) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CCAR1 Polyclonal Antibody detects CCAR1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: CCAR1 protein stained by CCAR1 Polyclonal Antibody (Product # PA5-78532) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CCAR1 Polyclonal Antibody detects CCAR1 protein at nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. CCAR1 Polyclonal Antibody (Product # PA5-78532) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CCAR1 Polyclonal Antibody detects CCAR1 protein at nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CCAR1 Polyclonal Antibody (Product # PA5-78532) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
