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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-28534 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Latexin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HeLaS3, Molt-4, mouse brain, rat brain, , rat heart. Predicted reactivity: Mouse (85%), Rat (85%), Rhesus Monkey (97%), Bovine (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Acute Sleep Loss Upregulates the Synaptic Scaffolding Protein, Homer1a, in Non-canonical Sleep/Wake Brain Regions, Claustrum, Piriform and Cingulate Cortices.
Zhu J, Hafycz J, Keenan BT, Guo X, Pack A, Naidoo N
Frontiers in neuroscience 2020;14:188
Frontiers in neuroscience 2020;14:188
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Latexin was performed by separating 50 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Latexin Polyclonal Antibody (Product # PA5-28534) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Latexin Polyclonal Antibody (Product # PA5-28534). Sample (30 µg whole cell lysate). A: 293T. B: HeLa S3. 12% SDS PAGE. Latexin Polyclonal Antibody (Product # PA5-28534) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Latexin was performed by separating 50 µg of rat tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Latexin Polyclonal Antibody (Product # PA5-28534) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Latexin Polyclonal Antibody (Product # PA5-28534). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. Latexin Polyclonal Antibody (Product # PA5-28534) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of LXN in paraformaldehyde-fixed mouse ESC D3 cells using a LXN polyclonal antibody (Product # PA5-28534) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using LXN (Product # PA5-28534) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 5 cFos levels increase in the claustrum, lateral hypothalamus (LHA), and locus coeruleus (LC) with 3 h sleep deprivation (SD) compared to sleeping control (SC) animals. (A) 3 h SC cFos staining; (A') 3 h sleep control with cFos and Latexin as marker for the claustrum; (B) 3 h SD cFos staining; (B') 3 h SD with cFos and Latexin; (C) 3 h SC cFos staining; (C') 3 h sleep control with cFos and Orexin as marker for the LHA; (D) 3 h SD cFos staining; (D') 3 h SD with cFos and Orexin; (E) 3 h SC cFos staining; (E') 3 h sleep control with cFos and tyrosine hydroxylase (TH) as marker for the LC; F) 3 h SD cFos staining; (F') 3 h SD with cFos and TH. Bar size A: 500 mum; C,E : 250 mum.
