PA5-28534

antibody from Invitrogen Antibodies

Targeting: LXN

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28534

Provider

Invitrogen Antibodies

Product name

Latexin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, HeLaS3, Molt-4, mouse brain, rat brain, , rat heart. Predicted reactivity: Mouse (85%), Rat (85%), Rhesus Monkey (97%), Bovine (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Acute Sleep Loss Upregulates the Synaptic Scaffolding Protein, Homer1a, in Non-canonical Sleep/Wake Brain Regions, Claustrum, Piriform and Cingulate Cortices.

Zhu J, Hafycz J, Keenan BT, Guo X, Pack A, Naidoo N
Frontiers in neuroscience 2020;14:188

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of LXN in paraformaldehyde-fixed mouse ESC D3 cells using a LXN polyclonal antibody (Product # PA5-28534) at a 1:200 dilution.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 5 cFos levels increase in the claustrum, lateral hypothalamus (LHA), and locus coeruleus (LC) with 3 h sleep deprivation (SD) compared to sleeping control (SC) animals. (A) 3 h SC cFos staining; (A') 3 h sleep control with cFos and Latexin as marker for the claustrum; (B) 3 h SD cFos staining; (B') 3 h SD with cFos and Latexin; (C) 3 h SC cFos staining; (C') 3 h sleep control with cFos and Orexin as marker for the LHA; (D) 3 h SD cFos staining; (D') 3 h SD with cFos and Orexin; (E) 3 h SC cFos staining; (E') 3 h sleep control with cFos and tyrosine hydroxylase (TH) as marker for the LC; F) 3 h SD cFos staining; (F') 3 h SD with cFos and TH. Bar size A: 500 mum; C,E : 250 mum.