Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-38890 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-COL12A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Targeted matrisome analysis identifies thrombospondin-2 and tenascin-C in aligned collagen stroma from invasive breast carcinoma.
Tomko LA, Hill RC, Barrett A, Szulczewski JM, Conklin MW, Eliceiri KW, Keely PJ, Hansen KC, Ponik SM
Scientific reports 2018 Aug 28;8(1):12941
Scientific reports 2018 Aug 28;8(1):12941
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Collagen XII alpha-1 in COS7 cells using a Collagen XII alpha-1 polyclonal antibody (Product # PA5-38890).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Collagen XII alpha-1 in COS7 cells using a Collagen XII alpha-1 polyclonal antibody (Product # PA5-38890).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Collagen XII alpha-1 in paraffin-embedded human skeletal muscle tissue using a Collagen XII alpha-1 polyclonal antibody (Product # PA5-38890).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Immunofluorescence validation of the four ECM proteins in the normal breast and IDC-s patient tissues. Immunofluorescence of normal breast and IDC-s patient tissues. Representative, immunostained images of ( a ) collagen XII, ( b ) fibronectin, ( c ) thombospondin-2, and ( d ) tenascin-C. These four ECM proteins were found localized near cellular (DAPI positive) regions in the normal breast tissues. In the IDC-s tissues, the four signature proteins associated with stromal/fibrous structures. Scale bar = 25 um. Normal breast, n = 8; IDC, n = 6.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Co-localization of aligned collagen fibers with THBS-2 and TNC in normal breast and IDC-s patient tissues. ( a ) Concentrations from quantitative LC-MS/MS analysis for collagen XII, fibronectin, thrombospondin-2, and tenascin-C. All four proteins are increased in abundance in IDC-c and IDC-s compared to normal tissue. Error bars represent sample variance, X represents the mean concentration. *p