Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP1-88062 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88062, RRID:AB_11036654
- Product name
- Rabbit Polyclonal Collagen XII alpha1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Collagen XII alpha1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Conditioning of 3D Printed Nanoengineered Ionic-Covalent Entanglement Scaffolds with iP-hMSCs Derived Matrix.
Sears C, Mondragon E, Richards ZI, Sears N, Chimene D, McNeill EP, Gregory CA, Gaharwar AK, Kaunas R
Advanced healthcare materials 2020 Aug;9(15):e1901580
Advanced healthcare materials 2020 Aug;9(15):e1901580
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Collagen XII alpha1 Antibody [NBP1-88062] - Analysis in human cell line SiHa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Collagen XII alpha1 Antibody [NBP1-88062] - Analysis in human cell lines U2OS and A-431 using Anti-COL12A1 antibody. Corresponding COL12A1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Collagen XII alpha1 Antibody [NBP1-88062] - Staining of human liver shows very weak cytoplasmic positivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Collagen XII alpha1 Antibody [NBP1-88062] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Collagen XII alpha1 Antibody [NBP1-88062] - Staining of human testis shows strong membranous positivity in peritubular myoid cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Collagen XII alpha1 Antibody [NBP1-88062] - Staining of human endometrium shows strong positivity in basement membranes.