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Western blot
ELISA
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunoprecipitation [2]
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Validation data
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- Product number
- LS-C670594 - Provider product page
- Provider
- LSBio
- Product name
- PDLIM4 / RIL Antibody LS-C670594
- Antibody type
- Polyclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Positive Western Blot detected in K562 whole cell lysate. All lanes: PDLIM4 antibody at 6.1 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 36, 26 KDa. Observed band size: 36 KDa
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Positive Western Blot detected in K562 whole cell lysate. All lanes: PDLIM4 antibody at 6.1 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 36, 26 KDa. Observed band size: 36 KDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating PDLIM4 in K562 whole cell lysate Lane 1: Rabbit control IgG instead of PDLIM4 Antibody in K562 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: PDLIM4 Antibody (6µg) + K562 whole cell lysate (500µg) Lane 3: K562 whole cell lysate (20µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating PDLIM4 in K562 whole cell lysate Lane 1: Rabbit control IgG instead of PDLIM4 Antibody in K562 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: PDLIM4 Antibody (6µg) + K562 whole cell lysate (500µg) Lane 3: K562 whole cell lysate (20µg)
