Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-9853-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IkB zeta Monoclonal Antibody (hft2nap), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The hft2nap monoclonal antibody reacts with human IkB zeta (INAP, MAIL, IkBz). IkBz is a member of the IkB family of cytoplasmic proteins and transcription factors and also shares structural homology with Bcl-3. It is induced in monocytes, macrophages and B cells in response to TLR and IL-1R signaling. In NK cells, IkBz is rapidly upregulated in response to IL-12 and IL-18 and is necessary for production of IFN gamma. In T cells, IkBz is not necessary for IFN gamma and IL-4 production by Th1 and Th2 cells, respectively. However in Th17 cells, IkBz acts in concert with ROR alpha and ROR gamma t to upregulate IL-17A, IL-17F, IL-21, IL-22 and IL-23R expression. Applications Reported: This hft2nap antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This hft2nap antibody has been pre-titrated and tested on stimulated human peripheral blood mononuclear cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- hft2nap
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references IκBζ augments IL-12- and IL-18-mediated IFN-γ production in human NK cells.
IκBζ is essential for natural killer cell activation in response to IL-12 and IL-18.
IkappaBzeta regulates T(H)17 development by cooperating with ROR nuclear receptors.
MAIL regulates human monocyte IL-6 production.
A novel role for IkappaBzeta in the regulation of IFNgamma production.
Kannan Y, Yu J, Raices RM, Seshadri S, Wei M, Caligiuri MA, Wewers MD
Blood 2011 Mar 10;117(10):2855-63
Blood 2011 Mar 10;117(10):2855-63
IκBζ is essential for natural killer cell activation in response to IL-12 and IL-18.
Miyake T, Satoh T, Kato H, Matsushita K, Kumagai Y, Vandenbon A, Tani T, Muta T, Akira S, Takeuchi O
Proceedings of the National Academy of Sciences of the United States of America 2010 Oct 12;107(41):17680-5
Proceedings of the National Academy of Sciences of the United States of America 2010 Oct 12;107(41):17680-5
IkappaBzeta regulates T(H)17 development by cooperating with ROR nuclear receptors.
Okamoto K, Iwai Y, Oh-Hora M, Yamamoto M, Morio T, Aoki K, Ohya K, Jetten AM, Akira S, Muta T, Takayanagi H
Nature 2010 Apr 29;464(7293):1381-5
Nature 2010 Apr 29;464(7293):1381-5
MAIL regulates human monocyte IL-6 production.
Seshadri S, Kannan Y, Mitra S, Parker-Barnes J, Wewers MD
Journal of immunology (Baltimore, Md. : 1950) 2009 Oct 15;183(8):5358-68
Journal of immunology (Baltimore, Md. : 1950) 2009 Oct 15;183(8):5358-68
A novel role for IkappaBzeta in the regulation of IFNgamma production.
Raices RM, Kannan Y, Bellamkonda-Athmaram V, Seshadri S, Wang H, Guttridge DC, Wewers MD
PloS one 2009 Aug 26;4(8):e6776
PloS one 2009 Aug 26;4(8):e6776
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were unstimulated (left) or stimulated with recombinant human IL-2 (Product # 14-8029-81), IL-12 (Product # 14-8129-62), and IL-18 (right) for 6 hours. Cultures were then harvested, surface-stained with Anti-Human CD56 (NCAM) APC (Product # 17-0567-42), and fixed and permeabilized with the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00). After fixation and permeabilization, cells were stained with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) or Anti-Human I kappa B Zeta PE. Cells in the lymphocyte gate were used for analysis and quadrant lines demark isotype control.
- Conjugate
- Yellow dye