Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF5049 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse 5T4 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse 5T4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human 5T4 is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
AAH58198- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The Stem Cell Marker Lgr5 Defines a Subset of Postmitotic Neurons in the Olfactory Bulb.
A Subtype of Olfactory Bulb Interneurons Is Required for Odor Detection and Discrimination Behaviors.
Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells.
Yu Y, Moberly AH, Bhattarai JP, Duan C, Zheng Q, Li F, Huang H, Olson W, Luo W, Wen T, Yu H, Ma M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Sep 27;37(39):9403-9414
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Sep 27;37(39):9403-9414
A Subtype of Olfactory Bulb Interneurons Is Required for Odor Detection and Discrimination Behaviors.
Takahashi H, Ogawa Y, Yoshihara S, Asahina R, Kinoshita M, Kitano T, Kitsuki M, Tatsumi K, Okuda M, Tatsumi K, Wanaka A, Hirai H, Stern PL, Tsuboi A
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Aug 3;36(31):8210-27
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Aug 3;36(31):8210-27
Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells.
Rosu-Myles M, She YM, Fair J, Muradia G, Mehic J, Menendez P, Prasad SS, Cyr TD
PloS one 2012;7(6):e38954
PloS one 2012;7(6):e38954
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- 5T4 in D3 Mouse Cell Line. 5T4 was detected in immersion fixed D3 mouse embryonic stem cell line using Sheep Anti-Mouse 5T4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5049) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm/membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of 5T4 in D3 Mouse Cell Line by Flow Cytometry. Retinoic acid-treated D3 mouse embryonic stem cell line was stained with Sheep Anti-Mouse 5T4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5049, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).
