Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-57803 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 5T4 Recombinant Rabbit Monoclonal Antibody (24GB1990)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 24GB1990
- Vial size
- 20 µL
- Concentration
- 1.12 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 5T4. Trophoblast glycoprotein expression in wild-type (WT) and trophoblast glycoprotein (TPBG) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 alpha serves as a loading control. The blot was incubated with 5T4 Recombinant Monoclonal Antibody (Product # MA5-57802) at a 1:5,000 dilution and HRP-conjugated goat anti-rabbit secondary antibody at a 1:20,000 dilution respectively.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 5T4. Total cell lysates (30 μg) from C2C12, H9c2, HAP-1, HeLa, HepG2, and HT-1080 cell lines were loaded and separated by SDS-PAGE. The blot was incubated with 5T4 Recombinant Monoclonal Antibody (Product # MA5-57802) at a 1:5,000 dilution and HRP-conjugated goat anti-rabbit secondary antibody at a 1:20,000 dilution respectively.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical staining of 5T4 in HAP-1 cells using 5T4 Recombinant Monoclonal Antibody (Product # MA5-57802) at a 1:1,1000 dilution. Nuclei were stained blue with DAPI; Trophoblast glycoprotein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of 5T4 expression in HAP-1 cells using 5T4 Recombinant Monoclonal Antibody (Product # MA5-57802) at a 1:2,000 dilution. Green, isotype control; red, Trophoblast glycoprotein.
