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- References [29]
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- Product number
- 60188-1-Ig - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#60188-1-Ig, RRID:AB_10859484
- Product name
- WTAP antibody
- Antibody type
- Monoclonal
- Description
- KD/KO validated WTAP antibody (Cat. #60188-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 4A10G9
- Vial size
- 20ul, 150ul
Submitted references Alteration of N6-Methyladenosine RNA Profiles in Cisplatin-Induced Acute Kidney Injury in Mice.
miR-139-5p Loss-Mediated WTAP Activation Contributes to Hepatocellular Carcinoma Progression by Promoting the Epithelial to Mesenchymal Transition.
METTL3-dependent RNA m6A dysregulation contributes to neurodegeneration in Alzheimer's disease through aberrant cell cycle events.
The pathological tissue expression pattern and clinical significance of m6A-regulatory genes in non-small cell lung cancer.
mTORC1 promotes cell growth via m6A-dependent mRNA degradation.
Suppression of m6A mRNA modification by DNA hypermethylated ALKBH5 aggravates the oncological behavior of KRAS mutation/LKB1 loss lung cancer.
Oncogenic AURKA-enhanced N(6)-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.
Hypoxia induces chemoresistance of esophageal cancer cells to cisplatin through regulating the lncRNA-EMS/miR-758-3p/WTAP axis.
Arginine methylation of METTL14 promotes RNA N6-methyladenosine modification and endoderm differentiation of mouse embryonic stem cells.
Tumor Suppressor microRNA-138 Suppresses Low-Grade Glioma Development and Metastasis via Regulating IGF2BP2.
Identification of WTAP-related genes by weighted gene co-expression network analysis in ovarian cancer.
Validation strategies for antibodies targeting modified ribonucleotides.
Direct RNA sequencing reveals m6A modifications on adenovirus RNA are necessary for efficient splicing.
Stabilization of ERK-Phosphorylated METTL3 by USP5 Increases m6A Methylation.
ANKLE1 N6 -Methyladenosine-related variant is associated with colorectal cancer risk by maintaining the genomic stability.
Alteration of N6-methyladenosine epitranscriptome profile in unilateral ureteral obstructive nephropathy.
Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection.
Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.
AMPK facilitates intestinal long-chain fatty acid uptake by manipulating CD36 expression and translocation.
Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d.
Synaptic N6-methyladenosine (m6A) epitranscriptome reveals functional partitioning of localized transcripts.
METTL3 regulates WTAP protein homeostasis.
RNA m6 A modification enzymes shape innate responses to DNA by regulating interferon β.
Region-specific RNA m6A methylation represents a new layer of control in the gene regulatory network in the mouse brain.
Overexpression of miR-29a reduces the oncogenic properties of glioblastoma stem cells by downregulating Quaking gene isoform 6.
WTAP Expression Predicts Poor Prognosis in Malignant Glioma Patients.
The m(6)A Methyltransferase METTL3 Promotes Translation in Human Cancer Cells.
Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.
A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation.
Li CM, Li M, Zhao WB, Ye ZC, Peng H
Frontiers in molecular biosciences 2021;8:654465
Frontiers in molecular biosciences 2021;8:654465
miR-139-5p Loss-Mediated WTAP Activation Contributes to Hepatocellular Carcinoma Progression by Promoting the Epithelial to Mesenchymal Transition.
Liu W, Gao X, Chen X, Zhao N, Sun Y, Zou Y, Guan Y, Yang L, Pei X, Wang G, Wang B, Li M, Song W
Frontiers in oncology 2021;11:611544
Frontiers in oncology 2021;11:611544
METTL3-dependent RNA m6A dysregulation contributes to neurodegeneration in Alzheimer's disease through aberrant cell cycle events.
Zhao F, Xu Y, Gao S, Qin L, Austria Q, Siedlak SL, Pajdzik K, Dai Q, He C, Wang W, O'Donnell JM, Tang B, Zhu X
Molecular neurodegeneration 2021 Sep 30;16(1):70
Molecular neurodegeneration 2021 Sep 30;16(1):70
The pathological tissue expression pattern and clinical significance of m6A-regulatory genes in non-small cell lung cancer.
Li Y, Zhang X, Cai J, Ren L, Liu B, Wu M, Lu W, Li R, Zhang C, Huang C, Tong J, Liu A, Zheng Y, Ren D, Guo Y, Huang Y
The journal of gene medicine 2021 Nov 9;:e3397
The journal of gene medicine 2021 Nov 9;:e3397
mTORC1 promotes cell growth via m6A-dependent mRNA degradation.
Cho S, Lee G, Pickering BF, Jang C, Park JH, He L, Mathur L, Kim SS, Jung S, Tang HW, Monette S, Rabinowitz JD, Perrimon N, Jaffrey SR, Blenis J
Molecular cell 2021 May 20;81(10):2064-2075.e8
Molecular cell 2021 May 20;81(10):2064-2075.e8
Suppression of m6A mRNA modification by DNA hypermethylated ALKBH5 aggravates the oncological behavior of KRAS mutation/LKB1 loss lung cancer.
Zhang D, Ning J, Okon I, Zheng X, Satyanarayana G, Song P, Xu S, Zou MH
Cell death & disease 2021 May 20;12(6):518
Cell death & disease 2021 May 20;12(6):518
Oncogenic AURKA-enhanced N(6)-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.
Peng F, Xu J, Cui B, Liang Q, Zeng S, He B, Zou H, Li M, Zhao H, Meng Y, Chen J, Liu B, Lv S, Chu P, An F, Wang Z, Huang J, Zhan Y, Liao Y, Lu J, Xu L, Zhang J, Sun Z, Li Z, Wang F, Lam EW, Liu Q
Cell research 2021 Mar;31(3):345-361
Cell research 2021 Mar;31(3):345-361
Hypoxia induces chemoresistance of esophageal cancer cells to cisplatin through regulating the lncRNA-EMS/miR-758-3p/WTAP axis.
Zhu ZJ, Pang Y, Jin G, Zhang HY, Wang WH, Liu JW, Tuo GX, Wu P, Yang Y, Wang ZQ, Wang K
Aging 2021 Jun 3;13(13):17155-17176
Aging 2021 Jun 3;13(13):17155-17176
Arginine methylation of METTL14 promotes RNA N6-methyladenosine modification and endoderm differentiation of mouse embryonic stem cells.
Liu X, Wang H, Zhao X, Luo Q, Wang Q, Tan K, Wang Z, Jiang J, Cui J, Du E, Xia L, Du W, Chen D, Xia L, Xiao S
Nature communications 2021 Jun 18;12(1):3780
Nature communications 2021 Jun 18;12(1):3780
Tumor Suppressor microRNA-138 Suppresses Low-Grade Glioma Development and Metastasis via Regulating IGF2BP2.
Yang Y, Liu X, Cheng L, Li L, Wei Z, Wang Z, Han G, Wan X, Wang Z, Zhang J, Chen C
OncoTargets and therapy 2020;13:2247-2260
OncoTargets and therapy 2020;13:2247-2260
Identification of WTAP-related genes by weighted gene co-expression network analysis in ovarian cancer.
Wang J, Xu J, Li K, Huang Y, Dai Y, Xu C, Kang Y
Journal of ovarian research 2020 Sep 30;13(1):119
Journal of ovarian research 2020 Sep 30;13(1):119
Validation strategies for antibodies targeting modified ribonucleotides.
Weichmann F, Hett R, Schepers A, Ito-Kureha T, Flatley A, Slama K, Hastert FD, Angstman NB, Cardoso MC, König J, Hüttelmaier S, Dieterich C, Canzar S, Helm M, Heissmeyer V, Feederle R, Meister G
RNA (New York, N.Y.) 2020 Oct;26(10):1489-1506
RNA (New York, N.Y.) 2020 Oct;26(10):1489-1506
Direct RNA sequencing reveals m6A modifications on adenovirus RNA are necessary for efficient splicing.
Price AM, Hayer KE, McIntyre ABR, Gokhale NS, Abebe JS, Della Fera AN, Mason CE, Horner SM, Wilson AC, Depledge DP, Weitzman MD
Nature communications 2020 Nov 26;11(1):6016
Nature communications 2020 Nov 26;11(1):6016
Stabilization of ERK-Phosphorylated METTL3 by USP5 Increases m6A Methylation.
Sun HL, Zhu AC, Gao Y, Terajima H, Fei Q, Liu S, Zhang L, Zhang Z, Harada BT, He YY, Bissonnette MB, Hung MC, He C
Molecular cell 2020 Nov 19;80(4):633-647.e7
Molecular cell 2020 Nov 19;80(4):633-647.e7
ANKLE1 N6 -Methyladenosine-related variant is associated with colorectal cancer risk by maintaining the genomic stability.
Tian J, Ying P, Ke J, Zhu Y, Yang Y, Gong Y, Zou D, Peng X, Yang N, Wang X, Mei S, Zhang Y, Wang C, Zhong R, Chang J, Miao X
International journal of cancer 2020 Jun 15;146(12):3281-3293
International journal of cancer 2020 Jun 15;146(12):3281-3293
Alteration of N6-methyladenosine epitranscriptome profile in unilateral ureteral obstructive nephropathy.
Li X, Fan X, Yin X, Liu H, Yang Y
Epigenomics 2020 Jul;12(14):1157-1173
Epigenomics 2020 Jul;12(14):1157-1173
Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection.
Gokhale NS, McIntyre ABR, Mattocks MD, Holley CL, Lazear HM, Mason CE, Horner SM
Molecular cell 2020 Feb 6;77(3):542-555.e8
Molecular cell 2020 Feb 6;77(3):542-555.e8
Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.
Zhang Y, Liu X, Liu L, Li J, Hu Q, Sun R
Medical science monitor : international medical journal of experimental and clinical research 2020 Feb 22;26:e919644
Medical science monitor : international medical journal of experimental and clinical research 2020 Feb 22;26:e919644
AMPK facilitates intestinal long-chain fatty acid uptake by manipulating CD36 expression and translocation.
Wu W, Wang S, Liu Q, Shan T, Wang X, Feng J, Wang Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Apr;34(4):4852-4869
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Apr;34(4):4852-4869
Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d.
Knuckles P, Lence T, Haussmann IU, Jacob D, Kreim N, Carl SH, Masiello I, Hares T, Villaseñor R, Hess D, Andrade-Navarro MA, Biggiogera M, Helm M, Soller M, Bühler M, Roignant JY
Genes & development 2018 Mar 1;32(5-6):415-429
Genes & development 2018 Mar 1;32(5-6):415-429
Synaptic N6-methyladenosine (m6A) epitranscriptome reveals functional partitioning of localized transcripts.
Merkurjev D, Hong WT, Iida K, Oomoto I, Goldie BJ, Yamaguti H, Ohara T, Kawaguchi SY, Hirano T, Martin KC, Pellegrini M, Wang DO
Nature neuroscience 2018 Jul;21(7):1004-1014
Nature neuroscience 2018 Jul;21(7):1004-1014
METTL3 regulates WTAP protein homeostasis.
Sorci M, Ianniello Z, Cruciani S, Larivera S, Ginistrelli LC, Capuano E, Marchioni M, Fazi F, Fatica A
Cell death & disease 2018 Jul 23;9(8):796
Cell death & disease 2018 Jul 23;9(8):796
RNA m6 A modification enzymes shape innate responses to DNA by regulating interferon β.
Rubio RM, Depledge DP, Bianco C, Thompson L, Mohr I
Genes & development 2018 Dec 1;32(23-24):1472-1484
Genes & development 2018 Dec 1;32(23-24):1472-1484
Region-specific RNA m6A methylation represents a new layer of control in the gene regulatory network in the mouse brain.
Chang M, Lv H, Zhang W, Ma C, He X, Zhao S, Zhang ZW, Zeng YX, Song S, Niu Y, Tong WM
Open biology 2017 Sep;7(9)
Open biology 2017 Sep;7(9)
Overexpression of miR-29a reduces the oncogenic properties of glioblastoma stem cells by downregulating Quaking gene isoform 6.
Xi Z, Wang P, Xue Y, Shang C, Liu X, Ma J, Li Z, Li Z, Bao M, Liu Y
Oncotarget 2017 Apr 11;8(15):24949-24963
Oncotarget 2017 Apr 11;8(15):24949-24963
WTAP Expression Predicts Poor Prognosis in Malignant Glioma Patients.
Xi Z, Xue Y, Zheng J, Liu X, Ma J, Liu Y
Journal of molecular neuroscience : MN 2016 Oct;60(2):131-6
Journal of molecular neuroscience : MN 2016 Oct;60(2):131-6
The m(6)A Methyltransferase METTL3 Promotes Translation in Human Cancer Cells.
Lin S, Choe J, Du P, Triboulet R, Gregory RI
Molecular cell 2016 May 5;62(3):335-345
Molecular cell 2016 May 5;62(3):335-345
Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.
Geula S, Moshitch-Moshkovitz S, Dominissini D, Mansour AA, Kol N, Salmon-Divon M, Hershkovitz V, Peer E, Mor N, Manor YS, Ben-Haim MS, Eyal E, Yunger S, Pinto Y, Jaitin DA, Viukov S, Rais Y, Krupalnik V, Chomsky E, Zerbib M, Maza I, Rechavi Y, Massarwa R, Hanna S, Amit I, Levanon EY, Amariglio N, Stern-Ginossar N, Novershtern N, Rechavi G, Hanna JH
Science (New York, N.Y.) 2015 Feb 27;347(6225):1002-6
Science (New York, N.Y.) 2015 Feb 27;347(6225):1002-6
A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation.
Liu J, Yue Y, Han D, Wang X, Fu Y, Zhang L, Jia G, Yu M, Lu Z, Deng X, Dai Q, Chen W, He C
Nature chemical biology 2014 Feb;10(2):93-5
Nature chemical biology 2014 Feb;10(2):93-5
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 60188-1-Ig(WTAP antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human cervical cancer using 60188-1-Ig(WTAP antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The WTAP antibody from Proteintech is a mouse monoclonal antibody to a recombinant protein of human WTAP. This antibody recognizes human,mouse,rat antigen. The WTAP antibody has been validated for the following applications: ELISA, WB, IHC analysis.
