V5193

antibody from NSJ Bioreagents

Targeting: WTAP KIAA0105, MGC3925, Mum2

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Antibody Data

Product number

V5193

Provider

NSJ Bioreagents

Product name

WT1-associated protein Antibody / WTAP

Antibody type

Monoclonal

Description

This highly specific WT1-associated protein antibody is suitable for use in Flow cytometry/Immunohistochemistry applications with human samples.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

PCRP-WTAP-1A4

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Aliquot the WT1-associated protein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human colon carcinoma tissue with WT1-associated protein antibody (clone PCRP-WTAP-1A4). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of PFA-fixed human HeLa cells with WT1-associated protein antibody (clone PCRP-WTAP-1A4) followed by goat anti-mouse IgG-CF488 (blue); Red = unstained cells.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using WT1-associated protein antibody (PCRP-WTAP-1A4). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.