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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-27764 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WTAP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat nuclear extract, K562 nuclear extract.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WTAP using 30 µg of MOLT4 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a WTAP polyclonal antibody (Product # PA5-27764) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of WTAP was performed by separating 30 µg of Jurkat whole cell and nuclear extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a WTAP Polyclonal Antibody (Product # PA5-27764) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of WTAP was performed by separating 30 µg of K562 whole cell and nuclear extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a WTAP Polyclonal Antibody (Product # PA5-27764) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of WTAP in paraformaldehyde-fixed HeLa cells using a WTAP polyclonal antibody (Product # PA5-27764) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using WTAP (Product # PA5-27764) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
