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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- HPA010550 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA010550, RRID:AB_1858866
- Product name
- Anti-WTAP
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human WTAP, Gene description: Wilms tumor 1 associated protein, Alternative Gene Names: KIAA0105, MGC3925, Validated applications: WB, IHC, ICC, Uniprot ID: Q15007, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references N6-methyladenosine RNA modification (m6A) is of prognostic value in HPV-dependent vulvar squamous cell carcinoma
Comprehensive immunohistochemical analysis of N6-methyladenosine (m6A) writers, erasers, and readers in endometrial cancer
Condic M, Thiesler T, Staerk C, Klümper N, Ellinger J, Egger E, Kübler K, Kristiansen G, Mustea A, Ralser D
BMC Cancer 2022;22(1)
BMC Cancer 2022;22(1)
Comprehensive immunohistochemical analysis of N6-methyladenosine (m6A) writers, erasers, and readers in endometrial cancer
Ralser D, Condic M, Klümper N, Ellinger J, Staerk C, Egger E, Kristiansen G, Mustea A, Thiesler T
Journal of Cancer Research and Clinical Oncology 2022;149(6):2417-2424
Journal of Cancer Research and Clinical Oncology 2022;149(6):2417-2424
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and WTAP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417661).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
- Sample type
- Human
