Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-47620 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD109 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD109 in lysates of U-87 MG human glioblastoma/astrocytoma cell line. Samples were incubated in CD109 polyclonal antibody (Product # PA5-47620) using a dilution of 0.5 µg/mL followed by HRP-conjugated Anti-Sheep IgG. A specific band was detected for CD109 at approximately 161 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD109 in 0.2 mg/mL lysates of U-87 MG human glioblastoma/astrocytoma cell line. Samples were incubated in CD109 polyclonal antibody (Product # PA5-47620) using a dilution of 20 µg/mL. A specific band was detected for CD109 at approximately 161 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD109 in immersion fixed paraffin-embedded sections of human squamous cell carcinoma. Samples were incubated in CD109 polyclonal antibody (Product # PA5-47620) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CD109 in A431 human epithelial carcinoma cell line. Samples were incubated in CD109 polyclonal (Product # PA5-47620) or isotype control antibody followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody.