MA5-33005

antibody from Invitrogen Antibodies

Targeting: ARSA

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-33005

Provider

Invitrogen Antibodies

Product name

Arylsulfatase A Monoclonal Antibody (4C10)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

4C10

Vial size

100 µg

Concentration

500 µg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry of Arylsulfatase A in Raji cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with Arylsulfatase A Monoclonal Antibody (4C10) (Product # MA5-33005) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Arylsulfatase A in ANA-1 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with Arylsulfatase A monoclonal antibody (Product # MA5-33005) at a dilution of 1 µg (per 1x10^6 cells), followed by 488 conjugated goat anti-mouse IgG (30 min at 20°C) using a 5-10 µg (per 1x10^6 cells) dilution.