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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-96796 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Myomegalin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MKGTDSGSCC RRRCDFGCCC RASRRAHYTP YRSGDATRTP QSPRQTPSRE RRRPEPAGSW AAAAEEEEAA AAATPWMRDY FAEDDGEMVP RTSHTAAFLS DTKDRGPPVQ SQIWRSGEKV PFVQTYSLRA FEKPPQVQTQ ALRDFEKHLN DLKKENFSLK LRIYFLEERM QQKYEASRED IYKRNIELKV EVESLKRELQ DKKQHLDKTW ADVENLNSQN EAELRRQFEE RQQETEHVYE LLENKIQLLQ EESRLAKNEA ARMAALVEAE KECNLELSEK LKGVTKNWED VPGDQVKPDQ YTEALAQRDK; Positive Samples: Mouse skeletal muscle; Cellular Location: Cytoplasm, Golgi apparatus, Nucleus, centrosome, cytoskeleton, microtubule organizing center
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.59 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of mouse skeletal muscle, using PDE4DIP Polyclonal antibody (Product # PA5-96796) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 60s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Myomegalin in extracts of mouse skeletal muscle using Myomegalin Polyclonal Antibody (Product # PA5-96796) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Myomegalin in paraffin-embedded rat heart using Myomegalin Polyclonal Antibody (Product # PA5-96796) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Myomegalin in paraffin-embedded human kidney using Myomegalin Polyclonal Antibody (Product # PA5-96796) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Myomegalin in paraffin-embedded mouse kidney using Myomegalin Polyclonal Antibody (Product # PA5-96796) at a dilution of 1:100.
