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LearnA02402-1
antibody from Boster Biological Technology
Targeting: TNFSF15
MGC129934, MGC129935, TL1, TL1A, VEGI, VEGI192A
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- A02402-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-TL1A/TNFSF15 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for TL1A/TNFSF15 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Human. TL1A/TNFSF15 information: Subcellular Localization: Membrane; Tissue Specificity: Specifically expressed in endothelial cells. Detected in monocytes, placenta, lung, liver, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine and colon.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of TL1A using anti-TL1A antibody (A02402-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates,Lane 2: rat kidney tissue lysates,Lane 3: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TL1A antigen affinity purified polyclonal antibody (Catalog # A02402-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TL1A at approximately 28KD. The expected band size for TL1A is at 28KD.
- Additional image
