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Learn500-P240-100UG
antibody from Invitrogen Antibodies
Targeting: TNFSF15
MGC129934, MGC129935, TL1, TL1A, VEGI, VEGI192A
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- ELISA [1]
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- Product number
- 500-P240-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TL1A Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: QLRAQGEASV QFQALKGQEF APSHQQVYAP LRADGDKPRA HLTVVRQTPT QHFKNQFPAL HWEHELGLAF TKNRMNYTNK FLLIPESGDY FIYSQVTFRG MTSECSEIRQ AGRPNKPDSI TVVITKVTDS YPEPTQLLMG TKSVCEVGSN WFQPIYLGAM FSLQEGDKLM VNVSDISLVD YTKEDKTFFG AFLL. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TL-1A. Anti-Human TL-1A-specific antibody was purified by affinity chromatography employing an immobilized Human TL-1A matrix. Sandwich ELISA: To detect Human TL-1A by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TL-1A (500-P240Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TL-1A. Western Blot: To detect Human TL-1A by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TL-1A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Anti-mitotic chemotherapeutics promote apoptosis through TL1A-activated death receptor 3 in cancer cells.
Qi C, Wang X, Shen Z, Chen S, Yu H, Williams N, Wang G
Cell research 2018 May;28(5):544-555
Cell research 2018 May;28(5):544-555
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA: To detect Human TL-1A by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of TL1A Polyclonal Antibody (Product # 500-P240-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech TL1A Polyclonal Antibody, Biotin (Product # 500-P240BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TL-1A.
